宁夏枸杞叶中总黄酮含量与抗氧化活性关系

Correlation Between the Total Flavonoid Contents and Antioxidant Activity of Lycium barbarum L.Leaves

为进一步开发宁夏枸杞叶资源,研究宁夏枸杞叶抗氧化活性部位中总黄酮含量与抗氧化活性间关系.以乙醇做提取溶剂,再以不同极性溶剂对醇提液进一步萃取分离;并用DPPH法(DPPH.)测定不同极性萃取部分的抗氧化能力,用分光光度法测定不同极性萃取部分中的总黄酮含量.结果表明,除石油醚萃取部位外,其他各萃取部分在一定质量浓度时,对DPPH自由基(DPPH.)清除率均达到90%,且与总黄酮含量有极显著的相关性(P<0.01),其中,正丁醇萃取部分中的总黄酮含量最高,达到77.32mg/g,其清除自由基ρ(SC50)=0.048 3mg/mL,活性与维生素C相当.可为枸杞叶的深度开发提供参考.

To study the correlation between the total flavonoid content and antioxidant activities of different solvent extracts from the leaves of Lycium barbarum L.,to provide the reference for further development and utilization of the leaves of Lycium barbarum L..Ethanol extract from the leaves of Lycium barbarum L.was fractioned by different solvents,the antioxidant activities of different solvent extracts were evaluated by DPPH,flavonoid content was determined by Colorimetry.Except of petroleum ether extracts,free radical scavenging rate of other solvent extracts in a certain concentration are up to 90%,and there are the significant correlations（P0.01） between DPPH free radical scavenging activities and the total flavonoid content.Total flavoniod content of n-Butanol extract is the highest with 77.32 mg/g,its free radical scavenging activity is equal to Vitamin C,SC50 value is 0.0483 mg/mL.