光棘球海胆3个地理亚群的遗传多样性AFLP分析

Genetic Diversity Analysis of Three Geographic Populations of Sea Urchin Strongylocentrotus nudus by AFLP

应用AFLP技术对光棘球海胆的大连长海县群体、大连旅顺群体及日本青森县群体进行遗传多样性分析。试验结果表明,4对引物组合扩增得到231个扩增位点,其中168个多态位点,多态位点总比例为72.73%;3个群体的遗传多样性指数分别为0.3020±0.1925、0.2995±0.1977和0.2945±0.1935;Shannon多样性指数分别为0.4390±0.2767、0.4344±0.2820和0.4298±0.2766;群体内遗传变异系数为0.2987±0.0366,群体间遗传分化系数为0.0230,说明群体内的遗传多样性比较丰富。3个群体之间的遗传距离无显著差异,遗传相似度基本一致,用UPGMA方法对3个群体的143个个体的聚类分析显示,3个群体的个体交互混杂在一起,3个群体间没有因为地理隔离而产生明显的遗传分化。

Amplified fragment length polymorphism（AFLP） was used to analyze the genetic diversity of three populations of sea urchin Strongylocentrotus nudus from Changhai（CH）,and Lvshun（LS） in Dalian,China and Aomori in Japan（QS）.Totally,231 loci were amplified by 4 primer pairs,of which 168 were polymorphic with polymorphic loci of 72.73%.The Nei＇s genetic diversities index was found 0.3020±0.1925,0.2995±0.1977 and 0.2945±0.1935 in the three populations,while the Shannon genetic diversity index was 0.4390±0.2767,0.4344±0.2820 and 0.4298±0.2766.The genetic variation coefficient was found to be 0.2987±0.0366 with in the populations and 0.0230 among the three populations,indicating that the genetic diversity was rich in the individuals within populations.No significant differences in the genetic distance or genetic identity were found in the three populations.The cluster analysis of 143 individuals by UPGMA method based on genetic distance revealed that the geographical isolation did not cause the significant genetic differentiations in the three populations.