不同组分壳聚糖温敏凝胶材料理化性能评价

Physicochemical property of thermosensitivitive chitosan hydrogel materials with different composition ratio

目的:评价不同组分壳聚糖温敏凝胶材料的理化性能。方法:先对壳聚糖粉高温高压消毒,然后壳聚糖溶液(0.1mol/L)和β-甘油磷酸钠(β-GP)溶液(1.83 mol/L)分别按照体积比9∶1和7∶1两种配比组分制备壳聚糖温敏凝胶。通过粘度变化、凝胶时间、溶胀率、降解率、微观结构等观察指标比较2种凝胶的性能差异。结果:凝胶时间9∶1组为(6.1±0.68)min,7∶1组为(4.98±0.5)min(P<0.05);初始粘度9∶1组为(9.95±0.40)Paos,7∶1组为(9.90±0.36)Paos(P>0.05),终粘度9∶1组为(50.05±1.06)Paos,7∶1组为(45.25±0.69)Paos(P<0.05);溶胀率9∶1组为(16.6±0.8)%,7∶1组为(16.9±0.7)%(P>0.05);含溶菌酶组的降解率(%)明显高于不含酶组(P<0.05),加酶组和不加酶组7∶1组降解均快于9∶1组(P<0.05)。扫描电镜结果显示9∶1组平均孔径为(1.01±0.62)μm,7∶1组的平均孔径为(0.79±0.44)μm(P>0.05)。结论:9∶1配比的壳聚糖温敏凝胶理化性能优于7∶1比例组。

Objective： To compare the physical and chemical properties of thermosensitivitive chitosan hydrogel materials with different composition ratio.Methods： Chitosan powder was autoclaved at high temperature.The chitosan solusion（0.1 mol/L） and β-glycerophosphate（β-GP） solusion（1.83 mol/L） were mixed according to the volume ratio at 9∶ 1 and 7∶ 1 respectively.The two kinds of gel were compared by viscosity change,gelation time,swelling ratio,biodegradation rate and micro-structure.Results： The gelation time of 7∶ 1 group and 9∶ 1 group was（4.98±0.5） min and（6.1±0.68） min（P0.05）,the initial viscosity were（9.90±0.36） and（9.95±0.40） Paos（P0.05）,the final viscosity（45.25±0.69） and（50.05±1.06） Paos（P0.05）,the swelling ratio（16.9±0.7）% and（16.6±0.8）%（P0.05）,respectively.The degradation rate（%） in the groups containing lysozyme was higher than that in the groups without lysozyme（P0.05）,in 7∶ 1 group was higher than in 9∶ 1 group no matter containing lysozyme or not（P0.05）.Scanning electron microscopy showed that the average pore size of both groups was similar（P0.05）.Conclusion： The physicochemical property of 9∶ 1 group is superior to that of 7∶ 1 group.