滚环扩增技术的原理及其应用的研究

Principle of rolling circle amplification and its discussion in application

滚环扩增是近年来发展起来的一种恒温核酸扩增方法。这种方法不仅可以直接扩增DNA和RNA,还可以实现对靶核酸的信号放大,灵敏度达到一个拷贝的核酸分子,因此,RCA技术在全基因组扩增、单核苷酸多态性、DNA芯片、蛋白质芯片等方面检测中具有很大的应用价值和潜力。

Rolling circle amplification（RCA） is a recently developed isothermal nucleic acids amplification method.This method can not only amplify DNA and RNA directly,but also allow the enlargement of signal from target nucleic acids with the sensitivity up to one copy of nucleic acids molecule.As a result,the RCA technique plays great values and potentials in whole genome amplification,single nucleotide polymorphism,DNA chips,protein chips and so on.