单克隆抗体P53及RasP21在腺性膀胱炎及膀胱黏膜鳞状上皮化生中的表达研究

Expression of P53,RasP21 in glandular cystitis and squamous metaplasia of the bladder mucosa

目的探讨腺性膀胱炎(GC)、膀胱黏膜鳞状上皮化生(SMBM)及合并两种病理改变(GC-SMBM)的患者膀胱黏膜中P53、RasP21的表达。方法 96例确定有异常病理改变并行手术治疗的患者标本,其中GC40例,SMBM 32例,GC-SMBM 24例,另取21例正常膀胱黏膜为对照组。利用免疫组织化学法检测活检标本中P53、RasP21的表达情况。结果 GC组及SMBM组P53及RasP21阳性率表达与对照组差异均无统计学意义(均>0.05),GC-SMBM组P53、RasP21及两者同时表达阳性表达率与对照组差异均有统计学意义(均<0.05)。结论 GC-SMBM患者恶变可能性较大,需积极治疗并密切随访。

Objective To investigate the relationship between expression of P53 and RasP21 in glandular cystitis（GC）,squamous metaplasia of the bladder mucosa（SMBM） and combination of glandular cystitis and squamous metaplasia of the bladder mucosa（GC-SMBM）.Methods Specimens of 96 patients who were diagnosed with bladder mucosa pathological changes were collected.Among all the specimens,40 cases were GC;32 cases were SMBM and 24 cases were GC-SMBM.Expressions of P53 and RasP21 were detected by immunohistochemistry.Twenty-one normal samples were served as the control group.ResultsThe positive expression of P53 and RasP21 in GC and SMBM group were not significantly different from those in the control group（all P0.05）.The positive expression rate of P53,RasP21 in GC-SMBM were obviously higher than Those in the control group（P0.05）.Conclusions GC-SMBM are easier to malignant transformation and closely follow-up is necessary.