椭圆叶花锚的利胆有效成分分离与鉴定

Isolation and identification of cholaneresis active ingredients from Halenia elliptica

目的通过药理实验确定椭圆叶花锚利胆作用的有效部位,并对其进行成分分离与鉴定。方法采用胆管引流法试验椭圆叶花锚的利胆作用,并确定了有效部位。再利用硅胶柱色谱、ODS、葡聚糖凝胶等色谱手段分离,通过1H-NMR、13C-NMR等波谱技术确定化合物的结构。结果从椭圆叶花锚利胆有效部位分离得到了9个化合物,分别为2,3,5-trimethoxy-1-O-primevero-syloxyxanthone(1)、2,3,4,5-etramethoxy-1-O-primeverosyloxyxanthone(2)、l,8-dihydroxy-3,5-dimethoxanthone(3)、2,3,4,5,7-pentamethoxy-1-O-primeverosyloxyxanthone(4)、2,3,4,7-etramethoxy-1-O-primeverosyl-oxyxanthone(5)、木犀草苷(6)、木犀草素(7)、齐墩果酸(8)、熊果酸(9)。结论其中化合物3为首次从椭圆叶花锚中分得。

Objective To investigate the chemical constituents from Halenia elliptica, which have been confirmed choleretic effect. Methods Biliary drainage method was used to test the choleretic effect of H. elliptica and the compounds were identified. The compounds were isolated by chromatography （silica gel, ODS, and HPLC） and identified on the basis of physicochemical constants and spectral analysis （1H-NMR and 13C-NMR）. Results Nine sesquiterpenoids were isolated and their structures were identified as 2,3,5-trimethoxy-1-O-primeveros-yloxyxanthone （1）, 2,3,4,5-etramethoxy-1-O-primeverosyloxyxanthone （2）, l,8-dihydroxy-3,5- dimethoxanthone （3）, 2,3,4,5,7-pentamethoxy-1-O-primeverosy-loxyxanthone （4）, 2,3,4,7-etramethoxy-1-O-primeverosyl- oxyxanthone （5）, cinaroside （6）, luteolin （7）, anolic acid （8）, and ursolic acid （9）. Conclusion Compound 3 is isolated from H. elliptica for the first time.