比较雷帕霉素和吡柔比星抑制膀胱癌T24细胞生长活性的实验研究

Comparison of the inhibitory effect of Rapamycin and Pirarubicin on bladder cancer T24 cell

目的对单独使用雷帕霉素(RPM)和吡柔比星(THP)抑制膀胱癌T24细胞生长活性的作用进行比较,探究雷帕霉素在膀胱肿瘤患者中的应用前景。方法建立空白对照组,将雷帕霉素和吡柔比星分别调整至10μg/mL、0.8μg/100μL,作用于膀胱癌T24细胞,处理24h后分别使用MTT法、流式细胞仪、RT-PCR和划痕法探索比较在此浓度下两种药物对膀胱癌T24细胞的影响;在体内实验,BALB/c裸鼠种植转移性人膀胱癌构建荷瘤模型。随机分为RPM(2mg/kg)、THP(0.015mg/cm2)组和空白对照组(生理盐水),观察RPM和TPH对肿瘤生长及转移的影响。结果 RPM和THP分别在10μg/mL、0.8μg/100μL质量浓度下均对膀胱癌T24细胞生长活性具有明显抑制作用,同时此浓度下RPM和THP抑制T24细胞增殖的作用无明显差异(P>0.05);RPM组下调血管生长因子(VEGF)的作用明显强于THP组(P<0.05);体内实验中,RPM和THP均显著抑制肿瘤的生长及转移。结论雷帕霉素(RPM)和吡柔比星(THP)均具有良好的抑制T24细胞生长的作用,但在此浓度下THP的细胞毒性较雷帕霉素更强,并且雷帕霉素(RPM)明显抑制膀胱癌T24细胞的生长活性的作用为治疗膀胱癌治疗提供了实验参考,表现出良好的临床应用前景。

Objective To compare the effects of Rapamycin（RPM） and Pirarubicin（THP） on inhibiting the growth and metastasis of bladder tumor T24 cell,and to investigate the therapeutic value of RPM by bladder instillation for patients with bladder tumor.Methods Human bladder tumor cell line T24 were divided into 3 groups,the control group,the RPM group and the THP group.The RPM group and the THP group were treated respectively with RPM（10ng/mL） and THP（0.8μg/100μL） for 24 h.The MTT method,Flow cytometry,RT-PCR and Scarification were applied to investigate the effect of RPM and THP on the proliferation of the T24 cells.In vivo experiment,BALB/L nude mouse planted the bladder tumor cell T24,treated by RPM（2 mg/kg） and TPH（0.05 mg/cm2） to observe the inhibtion effect on the tumor growth and metastases.Results Both RPM and THP could significantly inhibit the growth of bladder tumor cell T24,with no difference between the two groups（P0.05）.However,RPM was more significant in regulating the VEGF mRNA expression than THP（P0.05）.In vivo experiment showed that both RPM and THP could inhibit the growth and metastases of tumor cells.Conclusions THP and RPM can significantly inhibit the growth and metastasis of bladder tumor T24 cell,but the cytotoxicity of THP is stronger than that of RPM and RPM is more valuable for patients with bladder tumor.