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哮喘小鼠肺组织TRPM8与TRPA1 mRNA的表达 被引量：1

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摘要 目的观察哮喘小鼠肺组织TRPM8与TRPA1 mRNA的表达。方法 SPF级雌性Balb/c小鼠16只,随机分为哮喘组和对照组,每组8只。哮喘组应用卵蛋白(OVA)建立哮喘小鼠模型,末次激发后留取肺组织,采用荧光实时定量RT-PCR技术检测支气管肺组织中TRPM8与TRPA1的mRNA表达水平。结果哮喘组肺组织中TRPM8与TRPA1 mRNA水平分别为(0.619 1±0.213 1)和(0.272 2±0.167 1),明显高于对照组(0.347 1±0.221 1和0.107 9±0.041 5),差异有统计学意义(P<0.05)。结论哮喘小鼠肺组织TRPM8与TRPA1 mRNA的表达上调,可能是冷刺激诱发哮喘发作的分子机制。
作者 马洪明 刘升明 陈万群 吴艳玲 刘晓妍 陈丽
机构地区 暨南大学附属第一医院呼吸内科 暨南大学医学院生化教研室
出处 《广东医学》 CAS CSCD 北大核心 2012年第6期750-752,共3页 Guangdong Medical Journal
基金 广东省医学科研基金资助项目(编号:A2008363)
关键词 哮喘 瞬时受体电位 TRPM8 TRPA1
分类号 R562.25 [医药卫生—呼吸系统]
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参考文献14

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二级参考文献34

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广东医学
2012年 第6期

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