HPLC法测定氨酚伪麻片Ⅱ的溶出度

Determination of dissolution of Anfenweima tablets II by HPLC

目的建立高效液相色谱法测定氨酚伪麻片Ⅱ的溶出度试验方法。方法色谱柱为Symmertry C18柱(3.9mm×150mm,5μm);流动相为甲醇-0.75%醋酸溶液(20︰80);检测波长W为254nm。结果对乙酰氨基酚在8～80μg/mL范围内线性关系较好,r=0.9999,回收率为98.92%,RSD为1.20%。结论本方法简便、快速、准确、重复性好,可作为氨酚伪麻片Ⅱ的溶出度的检测方法。

Objective To establish HPLC method for detecting dissolution of Anfenweima tablets Ⅱ. Methods Symmertry C18 column（3.9mm×150mm,5μm） was used, the mobile phase was methanol -0.75% acetic acid （20：80）, the detection wavelength was 254 nm. Results The linear range of Paracetamol was 8-80 μg/mL, coefficient correlation r = 0.999 9, the average recovery of paracetamol was 98.92% with RSD 3.20% Conclusion The method is simple, rapid, accurate and re- peatable. It can be used for detecting dissolution of Anfenweima tablets Ⅱ.