山莨菪碱对过度训练大鼠肾小管上皮细胞Toll样受体4表达的影响

Effect of anisodamine on renal Toll-like receptor 4 expression in exhausted rats

目的评价山莨菪碱对过度训练大鼠肾小管上皮细胞Toll样受体4（TLR4）表达的影响。方法雄性sD大鼠48只，体重200～220g，采用随机数字表法，将其随机分为3组：正常对照组（C组，n=8）、力竭运动组（E组，n=24）和山莨菪碱组（A组，n=16）。采用大鼠游泳至力竭建立过度训练模型，A组于力竭运动前20min腹腔注射山莨菪碱10mg／kg后进行力竭运动。检测。肾组织细胞凋亡和肾小管上皮细胞TLR4蛋白、TLR4mRNA的表达水平。结果与C组比较，E组和A组细胞凋亡率升高，肾小管上皮细胞TLR4蛋白和TLR4mRNA表达上调（P〈0．05）；与E组比较，A组细胞凋亡率降低，肾小管上皮细胞TLR4蛋白和TLR4mRNA表达下调（P〈0．05）。结论山莨菪碱可通过下调过度训练大鼠。肾小管上皮细胞TLR4表达，抑制肾脏细胞凋亡。

Objective To evaluate the effect of anisodamine on expression of Toll-like receptor 4 （TLR4） in the kidneys of exhausted rats. Methods Forty-eight male SD rats weighing 200-220 g were randomly divided into 3 groups ： control group （group Ⅰ ） （ n = 8） ; exhaustion group （ group Ⅱ ） （ n = 24） and anisodamine group （group Ⅲ ） （n = 16）. In groups Ⅱ and Ⅲ exhaustion was induced by exhausting swimming and defined by （1） the animal sank to the bottom and stayed for 〉 10 s during swimming, （2） uncoordinated swimming, and （3） the animal lost escape/fighting reflex when placed on the table. In group m intraperitoneal anisodamine 10 mg/kg was administered at 20 min before swimming. The animals were sacrificed immediately （Tl ）, 6 h （T2 ） and 24 h （T3 ） after exhaustion respectively. Their kidneys were obtained for detection of apoptosis and determination of TLR4 protein （by immuno-histochemical staining） and mRNA （by RT-PCR）. Results Exhaustion significantly increased the number of apoptotic ceils and upegulated TLR4 protein and mRNA expression in group Ⅱ as compared with group Ⅰ , and anisodamine significantly attenuated the exhaustion-induced increase in the number of apoptotie cells and up-regulation of TLR（4 protein and mRNA expression in group Ⅲ as compared with group Ⅱ . Conclusion Anisodamine can decrease apoptosis in renal ceils in exhausted rats by down-regulating TLR4 expression.