奥曲肽对胰腺癌细胞增殖、凋亡和血管内皮生长因子表达的影响

The effects of Octreotide on proliferation、apoptosis and VEGF expression in human pancreatic cancer cell line

目的:探讨生长抑素类似物奥曲肽对胰腺癌细胞增殖、凋亡和血管内皮生长因子(VEGF)表达的影响。方法:分别加入不同浓度奥曲肽培养细胞24 h,采用四甲基偶氮唑蓝(MTT)法检测细胞增殖,原位末端转移酶标记法(TUNEL)检测细胞凋亡,实时荧光定量PCR(RT-PCR)法检测细胞VEGF mRNA表达。结果:随着奥曲肽浓度的升高,细胞增殖受到抑制,凋亡率升高,VEGFmNRA表达下降,与对照组比有显著性差异(P<0.05)。结论:奥曲肽可抑制胰腺癌细胞增殖、促进胰腺癌细胞凋亡和降低胰腺癌细胞VEGF的表达。

Objective： To study the effects of somatostatin analogue Octreotide on proliferation、apoptosis and VEGF expression in human pancreatic cancer cell line.Methods： Proliferation was measured by MTT assay.Apoptosis was detected by TUNEL method.The expression of VEGF was analyzed by RT-PCR.Results： Octreotide inhibited the growth and VEGF expression of SW1990,and promoted the apoptosis in a dose-dependent manner（P0.05）.Conclusion： Octreotide may be used to clinical treatment by promoting apoptosis、inhibiting proliferation and VEGF expression in pancreatic cancer patient.