高效液相色谱法测定脉络宁颗粒中绿原酸含量

Determination of chlorogenic acid in Mailuoning granules by HPLC

目的应用反相高效液相色谱法测定脉络宁颗粒中绿原酸的含量。方法以乙睛-0.4%磷酸溶液(13:87)为流动相,流速为1.0mL/min,检测波长为327nm。采用C18化学键合硅胶为固定相,在327nm进行测定。结果绿原酸在0.1～0.8μg范围内线性关系良好,平均回收率为99.52%。结论该方法简便、可靠、准确,可用于该制剂的质量控制。

Objective To determine the content of chlorogenic acid in Mailuoning granules by reversed-phase high performance liquid chromatography.Methods C18 column was used with the mixture of acetonitrile-0.4% Phosphoric acid solution（13：87） as the mobilephase at a flow rate of 1.0 mL/min with the detection wavelength at 327 nm.Results The method proved to be linear over therange of 0.1-0.8 μg.The recovery of the assay was 99.52%.Coclusion The method appears to be simple,reliable,accurate and can be used to the quality control of the preparation.