牛乳腺上皮细胞的分离培养

Isolation and culture of bovine mammary epithelial cells

为了对牛乳腺上皮细胞(MECs)进行分离、培养和鉴定,并研究细胞分泌功能,试验通过胶原酶消化法分离得到了牛乳腺上皮细胞,采用传代法对细胞进行纯化,对细胞标志蛋白进行免疫荧光染色鉴定,通过体外诱导和RT-PCR分析鉴定细胞的分泌功能。结果表明:分离到的牛乳腺上皮细胞具有典型乳腺上皮细胞的形态特征,表达广谱角蛋白,经诱导后可分泌β-酪蛋白。

To isolate, culture and identificatify the bovine mammary epithelial cells （ MECs）, studying the secrete function of the cells. The bo vine mammary epithelial cells isolated with collagenase Ⅲ were purified using the passage methods. The cell marker proteins were determined with immunofiuorescence staining, and the secrete function of MECs was identified by RT PCR and in vitro induction. The results showed that the isolated bovine MECs had the typical morphological features ,expressed a broad spectrum of keratins, and secreted β - casein after induc tion.