摘要
本文以香港巨牡蛎(Crassostrea hongkongensis)和太平洋牡蛎(C.gigas)为研究对象,进行牡蛎异源三倍体研究试验。在水温25℃条件下,香港巨牡蛎剥离卵子于海水中浸泡促熟1.5h后,与太平洋牡蛎精子受精,获得最高卵裂率为(24.40±3.79)%。受精后20min,采用盐度为10的低渗海水处理杂交受精卵25min,可获得68.65%的异源三倍体幼虫,孵化率为12.71%。异源三倍体幼虫的存活率和生长率均未表现出优势。受精后第9天,异源三倍体幼虫的存活率仅为(0.116±0.023)%,平均壳长为(103.50±0.85)(m;对照组的存活率和壳长分别为(42.17±2.74)%和(123.25±8.60)μm。幼虫在附着变态前死亡。
Prior to the allotriploid induction, the feasibility of fertilization between Cras-sostrea hongkongensis （Lain and Morton, 2003） eggs and C. gigas （Thunberg, 1973） sperm had been verified before. Results showed that cleavage rate of （24.40±3.79）% of the inter-specific hybrid can be received with the eggs immersed in 25℃ seawater for 1.5h. The zy-gotes treated for 25 rain with a hypotonic solution of 10 salinities at 20rain after fertilization can yield the highest triploidy rate of 68.65% and the hatchery rate of 12.71%. No statisti-cal advantages of growth rate and survival rate were found in allotriploid group compared with control group. The survival rate and shell length of allotriploid group were （0. 116±0. 023）% and （103.50±0.85）ptm at the 9th day after and shell length of control group were （42.17±2. riploid hybrid larvae died before metamorphism. insemination. However, the survival rate 74）% and （123.25±8.60）ttm. All allot-
出处
《海洋湖沼通报》
CSCD
北大核心
2012年第1期33-40,共8页
Transactions of Oceanology and Limnology
基金
国家重点基础研究发展计划项目(2010CB126406)
国家自然科学基金项目(30771622)
国家高技术研究发展规划项目(2006AA10A401)资助
关键词
牡蛎
异源三倍体
低渗诱导
香港巨牡蛎
太平洋牡蛎
Oyster
allotriploid
hypotonic treatment
Crassostrea hongkongensis
Cras-sostrea gigas
