生殖道沙眼衣原体体外经不同浓度阿奇霉素作用后超微结构的变化

Ultrastructural changes of genital tract Chlamydia trachomatis after treatment with azithromycin in vitro

目的初步探讨不同浓度阿奇霉素作用下沙眼衣原体的超微结构形态变化。方法采用McCoy细胞培养方法，在沙眼衣原体实验菌株（D／UW-3／Cx）体外培养过程中添加含阿奇霉素质量浓度为O．0667、0．1340、0．1900、0．2680、0．3330mg／L的培养液，不加阿奇霉素的培养液作为空白对照组。在透射电镜下观察McCoy细胞和沙眼衣原体超微结构的变化。结果在接种D／UW-3／Cx时加入阿奇霉素培养48h后，随阿奇霉素浓度的增加，透射电镜下可见包涵体内外囊泡增多；有异常增大的网状体，部分存在分裂异常，甚至网状体坏死崩解；原体数目减少，体积增大，外膜皱褶增多。阿奇霉素质量浓度为0．3330mg／L时见不到包涵体。结论阿奇霉素可引起沙眼衣原体外膜膨大，形成囊泡；网状体异常增大，甚至崩解；原体减少。

Objective To observe the ultrastructural changes of Chlamydia trachomatis after treatment with azithromycin. Methods The Chlamydia trachomatis laboratory strain （D/UW-3/Cx） was cultured in McCoy cells with or without the presence of azithromycin of 0.06671 0.1340, 0.1900, 0.2680 and 0.3330 mg/L for 48 hours. The uhrastructural changes of host cells and Chlamydia trachomatis were observed by transmission electron microscopy. Results After 48-hour culture, vesicles increased in number both inside and outside of the inclusion bodies with the rise in azithromycin concentration; there were abnormally large reticulate bodies, some of which experienced abnormal division and even necrosis or breakdown; the number of elementary bodies was decreased, while their size was enlarged, with a more wrinkled outer membrane. No inclusion bodies were seen when the concentration of＂ azithromycin was 0.333 mg/L. Conclusions Azithromycin can induce an increment in the outer membrane of Chlamydia trachomatis, formation of vesicles, abnormal enlargement or breakdown of reticulate bodies, and a decrease in elementary bodies.