L-肉毒碱对庆大霉素引起的急性肾衰竭的保护作用研究

Study on the protective effect of L-carnitine against gentamicin-induced acute renal failure

目的检测L-肉毒碱是否对庆大霉素诱导的急性肾衰竭具有保护作用。方法将成年雄性SD大鼠随机分为A、B、C、D 4组,各组连续8 d分别于腹腔内注射不同药物:A组注射生理盐水,B组注射庆大霉素[80 mg/(kg.d)],C组注射L-肉毒碱[200 mg/(kg.d)],D组依次注射L-肉毒碱[200 mg/(kg.d)]和庆大霉素[80 mg/(kg.d)],两次给药时间间隔1 h。各组大鼠分别于给药后第9天采集血液、尿液,检测生化指标,观察肾脏组织形态学改变。结果与A组和C组相比,B组大鼠血清尿素氮(BUN)、肌酐(Cr)、尿蛋白含量增加,尿液总量减少,差异有统计学意义(P<0.01、P<0.05)。与B组相比,D组血清BUN、Cr含量明显减少,尿蛋白减少,尿量增加,差异有统计学意义(P<0.05)。结论 L-肉毒碱对庆大霉素诱导的急性肾衰竭具有保护作用。

Objective To study whether L-carnitine has beneficial impact on gentamicin（GM）-induced acute renal failure（ARF）.Methods Adult male SD rats were assigned to four groups（A,B,C,D）： the rats of group A were injected normal saline for 8 consecutive days;the rats of group B were injected GM [80 mg/（kg·d）] for 8 days;the rats of group C were injected L-carnitine [200 mg/（kg·d）] for 8 days;the rats of group D were injected GM [80 mg/（kg·d）] 1 hour after L-carnitine [200 mg/（kg·d）] injection for 8 days.Then,the blood,urine were collected in the ninth day,the biochemical indicators were detected,and to observe the morphological changes of kidney tissue.Results Compared with group A and C,concentration of serum blood urea nitrogen（BUN）,creatinine（Cr） and urinary protein significantly increased and the total urine decreased in group B（P 〈 0.01,P 〈 0.05）.Compared with group B,concentration of BUN,Cr and urinary protein significantly decreased and urince increased in group D（P 〈 0.05）.Conclusion L-carnitine has protective impact on gentamicin-induced acute renal failure