摘要
目的探讨RAS蛋白在胶质瘤中的表达及其对人脑胶质瘤细胞生长的影响。方法用免疫组化染色法检测RAS蛋白在正常脑组织及各级别胶质瘤组织中的表达水平，并采用四甲基偶氮唑盐微量酶反应比色法和流式细胞技术检测降低RAS活性后U251细胞的增殖和凋亡情况；用Western印迹法检测ERK和AKT信号通路。结果胶质瘤组织中RAS的表达随胶质瘤恶性程度增高而增强。抑制RAS蛋白活性后，RAS信号通路的下游分子ERK和AKT蛋白磷酸化水平降低；U251胶质瘤细胞生长受抑制，细胞生长阻滞在G1期且凋亡增加。结论RAS蛋白在胶质瘤中高表达，抑制其活性可下调ERK和AKT信号通路，进而调控细胞的生长。
Objective To study the expression of RAS protein in human glioma tissues and its influence on tumor growth. Methods RAS protein expression in glioma tissues was determined by immunohistochemical （IHC） staining. Subsequently, MTT cell proliferation assay, flow cytometry and Western blotting were used to assay U251 cells with reduced RAS expression. Results The expression of RAS in glioma was increased and strongly correlated with pathological grade. Downregulation of RAS resulted in glioma cells growth suppression and increased apoptosis. Conclusion The expression level of RAS protein in human glioma was increased. Downregulation of RAS can inhibit glioblastoma cell growth through the RAS signal pathway.
出处
《中华医学遗传学杂志》
CAS
CSCD
北大核心
2012年第2期159-162,共4页
Chinese Journal of Medical Genetics
基金
基金项目：国家自然科学基金（81072078,30872657）
卫生部科研基金（W2011BX009）
江苏省自然科学基金（2010580,2008475）
江苏高校优势学科建设工程资助项目
南京医科大学第一附属医院创新团队工程
