茵陈多糖对梗阻性黄疸幼鼠肝脏纤维化的保护作用研究

Protective effects of Artemisia capillaries polysaccharides for hepatic fibrosis on immature rats with obstructive jaundice

目的探讨茵陈多糖对梗阻性黄疸幼鼠肝脏纤维化的保护作用。方法将Wistar幼龄大鼠40只随机分为四组:对照组、模型组、茵陈多糖高剂量组[0.8 mg/(kg.d)]、茵陈多糖低剂量组[0.4 mg/(kg.d)]。后三组采用胆总管结扎法制作梗阻性黄疸模型,高剂量组及低剂量组采用茵陈多糖干预,高剂量组胃灌茵陈多糖0.8 mg/(kg.d),低剂量组胃灌茵陈多糖0.4 mg/(kg.d),对照组、模型组胃灌生理盐水,并于术后2周分别测定各组幼鼠血清谷丙转氨酶(ala-nine aminotransferase,ALT)、谷草转氨酶(aspartate aminotransferase,AST)、总胆红素(total bilirubin,TBIL)、间接胆红素(indirect bilirubin,IBIL)、直接胆红素(ndirect bilirubin,DBIL)及肝脏组织层粘连蛋白(laminin,LA)、透明质酸酶(hyaluronidase,HA)、Ⅲ型前胶原(TypeⅢprocollagen,PC-Ⅲ)及Ⅳ型胶原(typeⅣcollagen,Ⅳ-C)表达水平,采用HE染色观察各组大鼠肝脏病例变化。结果高、低剂量组术后2周血清ALT、AST活性及TBIL、IBIL、DBIL水平低于模型组,高于对照组(P<0.05);低剂量组及高剂量组血清LA、HA、PC-Ⅲ及Ⅳ-C表达水平低于模型组,高于对照组(P<0.05),模型组肝脏组织肝小叶结构破坏,纤维组织增生明显,低剂量组及高剂量干预组肝小叶结构破坏及维组织增生较轻。结论茵陈多糖能够减轻梗阻性黄疸幼鼠肝脏病理损伤,减轻肝脏纤维化。

Objective To investigate the protect effects of Artemisia capillaries polysaccharides for hepatic fibrosis on im- mature rats with obstructive jaundice. Methods Forty rats immature were divided into four groups, control group, model group [0.4 mg/（kg-d）], Artemisia capillaries polysaccharides high-dose group [0.8 mg/（kg-d）] and Artemisia capillaries polysaccharides low-dose group, obstructive jaundice were made in immature rats by double-ligating bile duct in the mod- el group, high-dose group and low-dose group, rats in high-dose group and low-dose group were interfered with Artemisia capillaries polysaccharides 0.8 mg/（kg＇d） dose were taken in high-dose group and and 0.4 mg/（kg.d） dose were taken in low-dose group with intragastric administration, physiological saline were taken in control group and model group, at two weeks after operations, the levels of serum ALT, AST, TBIL, IBIL, DBIL and the level of LA, HA, PC-III and 1V-C in serum were detected. The change of liver texture were observed with HE stain. Results At 2 weeks after operations, levels of ALT, AST, TBIL, IBIL, DBIL in serum were lower in high-dose and low-dose groups than in model group, and was higher than in control group （P 〈 0.05）, the level of LA, HA, PC- m and IV-C was lower in high-dose and low-dose groups than in model group, and was higher than in control group （P 〈 0.05）, texture of hepatic lobule were damaged, fibroplasia was apparente in model group. The damage of hepatic lobule and fibroplasia was lighter in high-dose and low-dose groups than in model group. Conclusion Artemisia capillaries polysaccharides can decrease hepatic injury on immature rats with ob- structive jaundice and lessen hepatic fibrosis.