摘要
[目的]拟建立液相色谱串联质谱法测定质量分数为4%的春雷霉素可湿性粉剂中春雷霉素的分析方法。[方法]根据农药性质,优化仪器条件,以ACQUITY UPLC BEH AMIDE不锈钢柱为色谱柱;0.2%甲酸水和乙腈为流动相;流速：0.25 mL/min;应用UPLC-MS/MS选择多反应监测（MRM）正离子模式扫描测定。[结果]在0.002－0.1 mg/L范围内,春雷霉素线性关系良好（r2=0.9997）,回收率为97.2%－104.3%,相对标准偏差小于3.0%。[结论]方法精密度和准确度较高,是一种较为理想的检测分析方法。
[Aims] A analytical method of kasugamycin by UPLC-MS/MS was planed to be developed. [Methods] According to the properties of pesticides, instrumental conditions were systematically optimized, the samples were analyzed with ACQUITY UPLC BEH AMIDE stainless steel column, and 0.2% formic acid water solution-acetonitrile was used as the mobile phase with the flow rate at 0.25 mL/min, and data were acquired in multiple reactions monitoring mode （MRM）. [Results] In the linear range （0.002-0.1 mg/L）, there was a good linear relation and correlation coefficient （r2） of linearity was 0.9997, the corresponding recoveries were 97.2-104.3%, the relative standard deviations were lower than 3.0%. [Conclusions] The method was accurate and sensitive, which is a more ideal test analysis method.
出处
《农药》
CAS
北大核心
2012年第4期273-274,277,共3页
Agrochemicals
基金
国家"十二.五"科技支撑项目(2012BAK17B05)
