益气养阴消癥通络药对糖尿病肾病胃动力障碍大鼠胰高血糖素的影响

Effect of Yiqi Yangyin Xiaozheng Tongluo Drugs on Glucagon in Rat with Gastric Motility Disorder Induced by Diabetic Nephropathy

目的:观察益气养阴消癥通络中药对糖尿病肾病(DN)胃动力障碍大鼠胰高血糖素(Glucagon)的影响。方法:通过左肾切除术加链脲佐菌素腹腔注射诱导建立糖尿病肾病胃动力障碍大鼠模型,将其随机分为对照组、模型组、中药低剂量组、中药中剂量组、中药高剂量组。中药低、中、高剂量组大鼠分别给予益气养阴消癥通络5.29、10.58、21.16 g/kg.d-1灌胃,对照组及模型组给予等体积的生理盐水灌胃,共6周。禁食、禁水、麻醉、股动脉取血,分离血清后应用放射免疫法检测用药前后大鼠血清Glucagon的水平。结果:与对照组相比,模型组及中药各剂量组大鼠血Glucagon水平明显升高(P<0.05);与模型组相比,中药低、中、高各剂量组大鼠血Glucagon水平均有明显降低(P<0.05);中药各剂量组间比较差异无显著性(P>0.05)。结论:DN胃排空障碍大鼠存在胃肠激素紊乱,血清Glucagon水平明显升高,益气养阴消癥通络中药可明显降低DN胃排空障碍大鼠的血清Glucagon水平,此可能为其改善糖尿病肾病大鼠胃排空的机制之一。

Objective: to observe the effect of Yiqi Yangyin Xiaozheng Tongluo drugs on glucagon in rat with DN-induced gastric motility disorder.Methods: model rats with gastric motility disorder,made by left nephrectomy and intraperitoneal injection of streptozotocin,were divided into control group,model group,low,medium and high-dose groups.The latter three were given intragastric administration by 5.29,10.58 and 21.16 g/kg·d-1 while the former two given isovolumetric physiological saline respectively for 6 weeks.Do fasting,water deprivation,anesthesia,blood taking from femoral artery and serum separation before detecting glucagon level before and after administration with radio-immunity.Results: compared with control group,the glucagon increased significantly in model and drugs groups(P<0.05);compared with model group,the glucagon decreased significantly in drug groups(P<0.05);there was no significant difference between drug groups(P>0.05).Conclusion: rats with DN-induced gastric emptying disorder have symptoms of gastrointestinal hormone abnormality and glucagon increasing while the drugs can decrease the glucagon level which may be one of the mechanisms in improving the gastric emptying in rats with DN.