芬太尼和舒芬太尼对左旋甲状腺素钠预处理大鼠心肌保护作用的影响

Influences of fentanyl and sufentanil on the levothyroxine sodium preconditioning-induced cardioprotection in rats

目的评价芬太尼和舒芬太尼对左旋甲状腺素钠预处理大鼠心肌保护作用的影响。方法幼鼠采用随机数字表法随机分为7组（每组8只）：空白对照组（BC组）、对照组（c组）、左旋甲状腺素钠10μg组（10μg组）、芬太尼组（F组）、舒芬太尼组（S组）、芬太尼联合左旋甲状腺素钠组（F＋L组）和舒芬太尼联合左旋甲状腺素钠组（S＋L组）。采用Langendorff装置建立离体心脏缺血/再罐注损伤（ischemia／repeffusioninjury，I／RI）模型，F组和F＋L组灌注液中芬太尼浓度为30μg/L，S组和S＋L组灌注液中舒芬太尼浓度为3μg/L。结果再灌注30rain时血流动力学F组和S组明显差于BC组；10tang组、F＋L组和S＋L组优于C组、F组和S组。BC组、C组、10μg组、F组、S组、F＋L组和S＋L组再灌注期的冠脉流量分别是（19±2）、（16±1）、（21±2）、（17±1）、（17±1）、（21±1）、（22±1）ml／min，并且10μg组、F＋L组和S＋L组的冠脉流量高于C组、F组和S组。冠脉流出液中的肌酸激酶同工酶（creatinekinase，CK-MB）活性C组、F组和s组高于10μg组、F＋L组和S＋L组。心肌热休克蛋白70（heatshockproteins70，HSP70）和肌球蛋白重链（myosinheavy-chain，MHC）OtmRNA表达10μg组、F＋L组和S＋L组明显强于C组、F组和s组。但是所有检测指标在10μg组、F＋L组和S＋L组之间差异无统计学意义。结论芬太尼和舒芬太尼对左旋甲状腺素钠预处理的心肌保护作用均无明显影响。

Objective To evaluate influences of fentanyl and sufentanil on the cardioprotection effect of preemptive levothyroxine-sodium administration in the rats. Methods Immature rats were randomly allocated into seven groups （n=8）： blank control group （group BC）, control group （group C）, levothyroxine-sodium 10 μg group（group 10 μg）, fentanyl group （group F）, sufentanil group （group S）, combined fentanyl and levothyroxine-sodium group （group F＋L） and combined sufentanil and levothyroxine-sodium group（group S＋L）. The isolated heart model was established with a Langendorff apparatus. Hearts in the groups F and F＋L were perfused with KH fluid containing fentanyl 30μg/L, and hearts in the groups S and S＋L were perfused with KH fluid containing sufentanil 3 μg/L. Results The hemodynamic variables at 30 min of the reperfusion were inferior in group F and S than in group BC, but better in group 10 μg, group F＋L and group S＋L than in group C, F and S. The coronary perfusion flows in group BC, group C, group 10μg, group F, group S, group F＋L and group S＋L were （19±2）, （16±1）, （21±2）, （17±1）, （17±1）, （21±1）, （22±1） ml/min, respectively, with a significantly higher coronary perfusion flow in group 10μg, group F＋L and group S＋L than in group C, group F and group S. The myocardial bound creatine kinase （CK-MB） level in the coronary perfusion fluid during the reperfusion period was significantly higher in groups C, F and S compared with groups 10 μg, F＋L and S＋L. The expression of b oth heat shock proteins70（HSP70） and myosin heavy-chain（MHC） mRNA in the myocardium was significantly higher in groups 10μg, F＋L and S＋L than in groups C, F and S. However, there were no differences in all the observed parameters among groups 10 μg, F＋L and S ＋L. Conclusions Both fentanyl and sufentanil do not produce significant influence on the cardioprotective effect of levothyroxine sodium preconditioning.