早期干预对宫内感染脑损伤仔鼠脑组织neuritin表达的影响

Effect Early Intervention and Rehabilitation Training for Neuritin Expression of Brain Injuried Neonatal Rat caused by Intrauterine Infection

目的:本研究旨在探讨早期干预及康复训练对宫内感染致脑损伤仔鼠脑组织神经突起生长因子neuritin表达的影响。方法:30只Wistar大鼠在受孕后第17天和18天连续2天腹腔注射脂多糖(LPS)420ug/kg.d作为模型组,10只受孕大鼠在相同日龄注射等量无菌生理盐水作为对照组,随机选取模型组仔鼠100只,分为干预组(T)50只,非干预组(NT)50只;随机选取对照组仔鼠50只为NS组。干预组进行早期干预(触摸和丰富环境)和康复训练,非干预组和NS组不给任何干预。在仔鼠出生后24小时内随机选取模型组和对照组各5只行胎盘HE染色,观察胎盘病理变化情况,3组分别在1天、7天、14天、21天、28天随机选取10只取仔鼠脑组织行HE染色观察脑组织病理变化和免疫组化染色观察脑组织neuritin的表达情况。分别于14天、21天、28天行改良BBB评定和悬吊实验测定评分。结果:LPS组较NS组悬吊实验和改良BBB得分及neuritin表达均显著降低(p<0.05),其中T组显著高于NT组(p<0.05)。结论:早期干预后可提高脑neuritin的表达并能改善其运动功能。

Objective： To observe the effect of early intervention and rehabilitation training for the neuritin expression of brain injuried neonatal rat caused by intrauterine infection.Methods： 30 pregnant rats injected LPS（420ug/kg.d） in the abdominal at 17 th, 18th pregnant as LPS group,I0 pregnant rats injected equivalent saline at the same time as control group.Randomly selected 100 offspring in LPS group,divided into intervention group （T） 50,non-intervention group （NT） 50;randomly selected 50 offspring in control group as the NS group.Take early intervention （ touch and a rich environment）, and rehabilitation training in the T group,NT group and NS group did not give any interference. Randomly selected 5 offspring within 24 hours after birth both in control group and LPS group take HE staining of placenta to observe pathological changes, randomly selected 10 offspring in the three groups at ld, 7d, 14d, 21d, 28d took HE staining to observe pathological changes in brain tissue and took immunohistochemical staining of brain tissue to observe the expression of neuritin. Take suspension experiments and modified BBB measurement at 14d, 21d, 28d.Results： Compared with NS group,the suspension experiments and modified BBB measurement sores and the expression of neuritin of LPS group was significantly lower（p〈0.05）,and T group was significantly higher than NT group （p〈0.05）.Collclusions： Early intervention could enhance the expression of the neuritin in brain injuried young mice and could improve its motor function.