早期干预对脑损伤仔鼠脑组织中肝细胞生长因子表达的影响

Effect of Early Intervention for Hepatoeyte Growth Factor Expression of Cerebral Brain Injury Neonatal Rat

目的:研究早期干预对脑损伤后仔鼠脑组织中肝细胞生长因子(HGF)表达的影响。方法:给予孕17天的30只大鼠连续2天腹腔注射脂多糖(LPS)420μg/kg,10只腹腔注射等剂量无菌生理盐水作为对照组。随机选取LPS组仔鼠40只,分为干预组(T)20只与非干预组(NT)20只,生理盐水组(NS)仔鼠20只。仔鼠出生后1日,随机选取各组仔鼠各3只进行脑组织HE染色,并观察脑组织病理改变情况。LPS组和NS组仔鼠出生后均进行早期干预,分别在生后7日、14日、21日、28日的4个时间点行脑组织免疫组织化学染色,观察仔鼠脑组织中HGF的表达及运动功能情况。结果:(1)LPS组仔鼠出生后7日、14日、21日、28日脑组织HGF的表达量较对照组升高(P<0.01);(2)生后7日、14日、21日、28日干预组仔鼠脑组织HGF表达量较非干预组升高(P<0.01)。结论:孕鼠腹腔注射LPS后HGF的表达升高,早期干预可进一步提高其表达量并有效改善脑损伤仔鼠的运动功能。

Objective： To investigate the effect of early intervention for hepatoeyte growth factor （HGF）expression of cerebral brain injury neonatal rat.Methods： Thirty pregnant rats were consecutively injected with LPS （420 μg/kg） on seventeenth day gestation,ten pregnant rats were consecutively injected with saline as comparison. Respectively, selected LPS group 40 pups and saline group 20 pups.Take 1 day old pups to make pathology and immunohistochemistry. Immunohistochemistry were used to detect the expression of HGF at 7th,14th,21th,28th days. To observe the expression of HGF and motor function.Results： 1, LPS group at 7th, 14th,21th,28th days,the expression of HGF in brain tissue than the control group increased （P〈0.01）;2,The expression of HGF in brain tissue in the intervention group compared with non-intervention had increased（P〈0.01）. Conclusions： Early intervention could improve the expression of HGF and motor function of the newborn rats effectively.