肺鳞癌和腺癌组织E_2F-1和c-myc及p14^(ARF)蛋白表达临床病理学意义的探讨

Clinicopathological significance and expression of E_2F-1,c-myc and p14^(ARF) protein in squamous cell carcinoma and adenocarcinoma of lung

目的:研究E2F-1、c-myc和p14ARF蛋白在肺鳞癌及腺癌中的表达情况,探讨其在肺癌发生发展中的作用机制及其与临床病理学特征间的关系。方法:应用免疫组化二步法检测71例肺鳞癌、腺癌及相应癌旁组织中E2F-1、c-myc和p14ARF蛋白的表达情况。结果:肺鳞癌及腺癌组织中E2F-1和c-myc蛋白的阳性表达率分别为83.1%(59/71)和81.7%(58/71),高于癌旁组织中的8.5%(6/71),P<0.05。p14ARF蛋白的阳性表达率为52.1%(37/71),低于相应癌旁组织的74.6%(53/71),P<0.05。并与患者年龄、性别、肿瘤大小、组织分化、病理分型、有无淋巴结转移及TNM分期无关,P>0.05。E2F-1、c-myc和p14ARF蛋白表达经相关分析显示两两相关,P<0.05。结论:肺鳞癌及腺癌内可见E2F-1和c-myc蛋白的过表达及p14ARF蛋白的表达缺失,可能与癌的发生发展有关。

OBJECTIVE： To investigate the expressions and clinicopathological characteristics of E2F-1,c-myc and p14ARF protein in the squamous carcinoma and adenocarcinoma of lung.METHODS：The expressions of E2F-1,c-myc and p14ARF protein of 71 cases of squamous cell carcinoma and adenocarcinoma of lung,and the related peritumoral lung tissues were detected by using immunohistochemical technique.RESULTS：The positive rates of E2F-1 and c-myc protein expression were 83.1%（59/71） and 81.7%（58/71） in cancerous tissues,which were significantly higher than 8.5%（6/71） in peritumoral lung tissues（P0.05）.And the positive expression of p14ARF protein in cancerous tissues（52.1%） was lower than which in the peritumoral tissues（74.6%,P0.05）.The expression rates of E2F-1,c-myc and p14ARF protein were no correlated with age,gender,tumor size,histological type,differentiation degree,lymphnode metastasis and TNM stage of patients（P0.05）.And there were significant correlation in any two of three indicators of E2F-1,c-myc and p14ARF protein expressions by correlation analysis（P0.05）.CONCLUSION： Abnormal expression of E2F-1,c-myc and p14ARF protein may play an important role in the tumorigenesis and progression for the squamous cell carcinoma and adenocarcinoma of lung.