乳清酸磷酸核糖转移酶和二氢嘧啶脱氢酶mRNA在胃癌中的表达及意义

Expressions and significance of orotate phosphoribosyl transferase and dihydropyrimidine dehydrogenase mRNA in gastric cancer tissues

目的检测5-氟尿嘧啶代谢相关酶乳清酸磷酸核糖转移酶（OPRT）和二氢嘧啶脱氢酶（DPD）mRNA在胃癌中的表达，探讨其与临床病理因素的关系。方法回顾性分析2007年6月至2008年11月内蒙古医学院附属医院收治的53例胃癌患者的临床资料。收集患者手术切除的胃癌组织及癌旁正常组织标本，应用RT．PCR法检测OPRT和DPD的mRNA表达，分析其与临床病理特征的关系。两组问比较采用配对样本t检验，多组间比较采用单因素方差分析。结果半定量RT-PCR检测结果显示，OPRTmRNA在胃癌组织中的表达为1．15±0．56，明显高于癌旁正常组织中的0．88±0．31，两者比较，差异有统计学意义（t=3．66，P〈0．05）；而DPDmRNA在胃癌组织和癌旁正常组织中的表达分别为0．95±0．50和0．90±0．41，两者比较，差异无统计学意义（t=0．68，P〉0．05）。胃癌组织中OPRTmRNA在无淋巴结转移的胃癌组织中表达为1．42±0．54，比在有淋巴结转移的胃癌组织中表达的1．00±0．52明显增高（t=7．94，P〈0．05）。DPDmRNA在高中分化、低分化腺癌和黏液腺癌中的表达逐渐增高，分别为0．67±0．36、0．93±0．24、1．58±0．38，3者比较，差异有统计学意义（F=27．71，P〈0．05）。结论OPRTmRNA在胃癌组织中的表达比相应癌旁组织表达量高，在有淋巴结转移的胃癌组织中比无淋巴结转移的胃癌组织中的表达降低；DPDmRNA在肿瘤组织中随组织学分型和组织分化程度降低而升高，在黏液腺癌中表达量最高。

Objective To detect the expressions of orotate phosphoribosyl transferase （OPRT） and dihy- dropyrimidine dehydrogenase （DPD） mRNA in gastric cancer tissues, and investigate their relationship with the clinicopathological factors. Methods The gastric cancer tissues and adjacent normal tissues were collected from 53 patients with gastric cancer at the Affiliated Hospital of Inner Mongolia Medical College from June 2007 to November 2008. The mRNA expressions of OPRT and DPD were detected by reverse transcriptional-polymerase chain reaction, and the correlation between the mRNA expressions of OPRT and DPD and the clinicopathological factors was analyzed. All data were analyzed by using the t test and the one-way analysis of variance. Results The mRNA expression of OPRT in the gastric cancer tissue was 1.15 ± 0.56, which was significantly higher than 0.88±0.31 in the adjacent normal tissues （t =3. 66, P 〈0.05）. The mRNA expressions of DPD in gastric cancer tissues and the adjacent normal tissues were 0.95 ± 0.50 and 0.90 ±0.41, respectively, with no significant difference between the 2 groups （t = 0. 68, P 〉 0.05）. The mRNA expressions of OPRT in the gastric cancer tissues with no lymph node metastasis was 1.42 ± 0.54, which was significantly higher than 1.00 ±0.52 in the gastric cancer tissues with lymph node metastasis （ t = 7.94, P 〈 0.05 ）. Poorly differentiated adenocarcinoma （ 0.93 ±0.24 ） and mucinous adenocarcinoma （ 1.58 ±0.38 ） showed a significantly higher DPD mRNA expression than moderately differentiated or well differentiated adenocarcinoma （ 0. 67±0. 36） （ F = 27. 71, P 〈 0.05 ）. Conclusions The mRNA expression of OPRT in gastric cancer tissue is higher than that in the adjacent normal tissues, and its expression in patients with lymph node metastasis is lower than that in patients without lymph node metastasis.Poorly differentiated adenocarcinoma showed higher DPD mRNA expression than moderately or well differemiated adenocareinoma, and its expression is highest in mucinous adenoearcinoma.