绍兴市无偿献血者中隐匿性HBV感染的基因型别及突变类型分析

Genotyping and mutational analysis of occult hepatitis B virus infection in blood donors of Shaoxing

目的 了解绍兴市无偿献血者隐匿性乙肝病毒感染情况及病毒的分子生物学特征.方法 应用ELISA的方法对绍兴市中心血站8692例无偿献血者进行筛查,对HBsAg阴性标本进行HBV DNA检测,从而检出隐匿性乙肝病毒感染者,再对阳性标本进行序列分析和氨基酸突变分析,从而了解绍兴市无偿献血者隐匿性乙肝病毒感染情况和病毒分子生物学特征,探讨隐匿性乙肝病毒感染的可能机制.结果 在总共8692例无偿献血者标本中,共计H BsAg（-）8644例,在8644例HBsAg（-）标本中,有8例HBV DNA阳性,隐匿性HBV感染比例为0.92‰（8/8692）,其中基因C型6例（75％）,基因B型2例（25％）；氨基酸突变分析显示有7株OBI病毒株在“a”表位发生特异性突变.结论 绍兴市无偿献血者中存在一定比例隐匿性乙肝病毒感染,隐匿性乙肝病毒感染与病毒基因突变有相关性.

Objective To assess the molecular biological characteristics of occult hepatitis B virus （ HBV ） infected blood donors in Shaoxing.Methods 8692 blood donors were screened using ELISA.The occult HBV infection was determined by DNA analysis among the HBsAg negative subjects.DNA sequencing and mutational analysis were further performed in the HBV DNA positive samples.The overall situation of occult HBV infection was hereby evaluated and the possible underlying mechanisms discussed.Results Among the 8644 HBsAg negative subjects out of 8692 blood donors,8 were HBV DNA positive.The occult HBV infection rate was 0.92‰（8/8692）.Among the 8 samples,6 were genotype C （75%） and 2 genotype B （25%）.In addition,a specific mutation in ＂a＂ epitope was observed in 7OBI virus strains by amino acid analysis.Conclusion There were occult HBV infected among blood donors in Shaoxing,which is probably associated with the gene mutation of HBV virus.