HPLC测定地锦草中没食子酸、槲皮素及山柰素含量

Determination of Gallic Acid,Quercetin and Kaempferol in Euphorbia humifusa Herba by HPLC

目的:建立同时测定地锦草药材中槲皮素、山柰素和没食子酸含量的高效液相色谱法。方法:采用Agilent TC-C18(4.6 mm×150 mm,5μm)柱分离,以甲醇和0.4%磷酸水溶液进行梯度洗脱,柱温30℃,流速1.0 mL.min-1。结果:没食子酸在0.051 8～0.621 6μg线性关系良好,平均回收率在102.17%(RSD 2.83%),槲皮素在0.070 2～0.702 0μg线性关系良好,平均回收率在97.80%(RSD 2.42%),山柰素在0.020 4～0.408 0μg线性关系良好,平均回收率在98.02%(RSD2.72%)。结论:操作简便,准确度高,重复性好,可作为地锦草质量控制方法之一。

Objective：To develop a HPLC method for determining quercetin,kaempferol and gallic acid in Euphorbia humifusa herba.Method： The analysis was performed on a column Agilent TC-C18（4.6 mm×150 mm,5 μm）,the solvent system composed of methanol-0.4% phosphoric.The column temperature was at 30 ℃,the flow rate was 1.0 mL·min-1.Result： Quercetin,kaempferol and gallic acid were linear in the range of 0.070 2-0.702 0,0.020 4-0.408 0,0.051 8-0.621 6 μg.The average recovery of quercetin,kaempferol and gallic acid was 97.80%（RSD 2.42%）,98.02%（RSD 2.728%）,102.17%（RSD 2.83%）.Conclusion： The method is quick,simple,and reproducible,which can be used to control the quality of Euphorbia humifusae herb.