HPLC测定复方昆丹胶囊中芍药内酯苷、芍药苷、二苯乙烯苷、五没食子酰葡萄糖

HPLC Analysis of the Composition of Albiflorin,Paeoniflorin, 2,3,5,4′-Tetrahydro Xystilbene-2-O-β-D-Glucoside and Penta-O-Galloyl-β-D-Glucoside in Kundan Capsule

目的:建立昆丹胶囊中芍药内酯苷、芍药苷、二苯乙烯苷、五没食子酰葡萄糖的含量测定方法。方法:采用Agilent TC-C18柱(4.6 mm×250 mm,5μm),以乙腈-甲醇-0.1%磷酸为流动相进行梯度洗脱,检测波长230 nm,柱温30℃,流速1.0 mL.min-1。结果:芍药内酯苷、芍药苷、2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷及五没食子酰葡萄糖浓度分别在0.02～1.84,0.03～1.65,0.03～3.44,0.01～0.81 mg(r>0.999 9)线性关系良好。低、中、高3个浓度的平均加样回收率分别为99.5%～102.6%(RSD 1.3%～2.1%),98.5%～103.5%(RSD 0.5%～2.4%),98.3%～104.2%(RSD 0.5%～2.2%)。结论:方法准确,灵敏,能有效测定昆丹胶囊中芍药内酯苷、芍药苷、2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷及五没食子酰葡萄糖成分的含量。

Objective：To establish an HPLC method for the simultaneous determination of albiflorin,paeoniflorin,2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside and penta-O-galloyl-β-D-glucoside in Kundan Capsule.Method： The analysis was carried out on an Agilent TC-C18 column（4.6 nm×250 mm,5 μm）.The mobile phase was composed of acetonitrile（A）-methanol（B）-0.1% phosphoric acid aqueous（C） with gradient elution;the detection wavelength was set at 230 nm;the column temperature was at 30 ℃;the flow rate was 1.0 mL·min-1.Result： The calibration curves of albiflorin,paeoniflorin,2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside and penta-O-galloyl-β-D-glucoside were linear in the ranges of 0.02-1.84,0.03-1.65,0.03-3.44,0.01-0.81 mg（r0.999 9） respectively.The mean recoveries of low,middle and high concentration of the four components were among 99.5%-102.6%（RSD 1.3%-2.1%）,98.5%-103.5%（RSD 0.5%-2.4%）,98.3%-104.2%（RSD 0.5%-2.2%）.Conclusion： The method is accurate and sensitive,which can be used in the quality control of Kundan capsule.