葛根芩连汤及其药效组分红外光谱表征分析

FT-IR Characteristic Analysis of Gegenqinlian Decoction and its Active Components Alignments

目的:研究葛根芩连汤及其药效组分的红外指纹表征,为建立复方组分指纹鉴定法提供科学依据。方法:应用傅里叶红外光谱(Foruier transform infrared,FT-IR)进行测定,采用复方汤剂和化学药效组分指纹表征表述质量特征。结果:葛根芩连汤的主要特征峰出现在1 727,1 614,1 509,1 448,1 362,1 272,1 031,838,767,582 cm-1等,安徽产区药材煎煮全方与道地药材煎煮全方的相似度最高。葛根芩连汤药效组分主要指纹表征(1 800～400 cm-1)有1 726,1 607,1 509,1 451,1 364,1 238,1 037,891,834,591 cm-1等。结论:采用红外指纹表征鉴别法可评价葛根芩连汤的质量,可反映出本质的主要特征,具有明显的专属性。

Objective：To study the foruier transform infrared（FT-IR） fingerprinting characteristics of Gegen Qinlian decoction and its active components alignments.Method： FT-IR was adopted to analyze and describe the characteristics by Gegen Qinlian decoction and the active components alignments.Result： The main characteristic peaks of Gegen Qinlian decoction are at 1 727,1 614,1 509,1 448,1 362,1 272,1 031,838,767,582 cm-1,etc.,and the crude drugs for decoction in Anhui region has the highest correlation coefficient with genuine medicinal materials.The FT-IR fingerprint characterization（1 800-400 cm-1） of the active components alignments for Gegen Qinlian decoction are 1 726,1 607,1 509,1 451,1 364,1 238,1 037,891,834,591 cm-1,etc.Conclusion： The FT-IR fingerprint identification method can be used for quality evaluation for Gegen Qinlian decoction,and reflect the main features.It has obvious specificity.