消渴丸中药成分对α-葡萄糖苷酶抑制作用的研究

The Inhibiting Effect of Compositions from Xiaoke Pill on the α-glucosidase

目的:研究消渴丸中药成分对大鼠肠黏膜α-葡萄糖苷酶是否有抑制作用,以探讨消渴丸中药成分的降糖作用机制。方法:用大鼠小肠上段提取α-葡萄糖苷酶,1∶5稀释使酶反应速度达到试剂盒测定要求。以10 g.L-1蔗糖作为底物,1 g.L-1阿卡波糖为阳性对照,分别加入不同浓度的消渴丸全药、消渴丸中药和格列本脲,37℃反应40 min后,在550 nm波长下测定吸光度(A)。通过测定反应体系中葡萄糖生成量并计算酶活性的抑制率来判断消渴丸中药成分对大鼠肠黏膜α-葡萄糖苷酶活性的影响。结果:阿卡波糖(1 g.L-1)对α-葡萄糖苷酶的抑制率为86.3%。消渴丸中药成分(2～200 mg.L-1)对α-葡萄糖苷酶有一定的抑制作用(抑制率18.8%～35.7%)。结论:消渴丸中药成分对大鼠小肠黏膜α-葡萄糖苷酶具有一定抑制作用。

Objective：To study whether the composition from Xiaoke pill has the inhibiting effect on the α-glucosidase,and find out the mechanism of reducing the blood glucose l.Method： The α-glucosidase was prepared from the upper part of rat small intestine and diluted by 5 times in order to reach the requirement of the kit.Sucrose（10 g·L-1） was used as the substrate,and acarbose（1 g·L-1） as the positive control.Different concentrations of Xiaoke pill,the composition and glibenclamide were added into the reaction system.the absorbance of the system at 550 nm was measured after reacting for 40 min under the temperature of 37 ℃.The α-glucosidase activity was detected by measuring the concentration of glucose produced.The inhibition ratio was calculated.Result： Acarbose（1 g·L-1） was shown to be an α-glucosidase inhibitor with an evident inhibition ratio（86.3%）.The composition（2-200 mg·L-1） had an inhibition ratio of 18.8%-35.7%.Conclusion： The composition from Xiaoke pill has some inhibiting effect on the α-glucosidase of rat small intestine.