柴朴汤对支气管哮喘大鼠气道重塑及肺组织中α-平滑肌肌动蛋白表达的影响

Investigation of Effect of Chaipu Decoction on Expression of α-smooth Muscle Actin and Airway Remodeling in Asthmatic Rats

目的:观察支气管哮喘大鼠气道重塑和α-平滑肌肌动蛋白(α-SMA)在哮喘大鼠模型中的动态表达及柴朴汤的干预作用。方法:将70只SD大鼠随机分为对照组、哮喘模型组、柴朴汤干预组,用卵蛋白(OVA)致敏激发建立大鼠哮喘模型,采用HE染色的方法及逆转录聚合酶链反应(RT-PCR)、免疫组织化学方法研究肺组织气道形态学参数的改变及α-SMA的表达。结果:①哮喘2,4,8周组总管壁面积(WAt/Pbm)、内壁面积(WAi/Pbm)及平滑肌面积(Wam/Pbm)均高于对照组(P<0.05),且随着激发天数的增加,上述指标有增加的趋势,哮喘4周组高于2周组,哮喘8周组高于4周组(均P<0.05);柴朴汤2,4,8周组,哮喘2,4周组WAt/Pbm,WAi/Pbm,Wam/Pbm均低于哮喘8周组(均P<0.05),但柴朴汤组高于对照组。②哮喘2,4,8周组的α-SMA的mRNA和蛋白水平明显高于柴朴汤2,4,8周组及对照组(均P<0.05);α-SMA的mRNA和蛋白水平随激发天数的增加而明显增加(均P<0.05);柴朴汤2周组的α-SMA的mRNA和蛋白水平与对照组相比,差异没有显著性;柴朴汤各周组与哮喘各相应周组比较,差异有显著性(P<0.05)。结论:哮喘大鼠肺组织α-SMA呈动态过量表达。柴朴汤可通过下调α-SMA表达,抑制气道平滑肌收缩及增生抑制气道重构。

Objective：To observe the effect of Chaipu decoction on ovalbumin（OVA）-induced asthma in rats and the dynamic expression of alpha-smooth muscle actin（α-SMA）.Method：Seventy sprague dawley（SD） rats were randomly divided into control group,model group（two weeks,four weeks and eight weeks）,chaipu decoction-treated group（two weeks,four weeks and eight weeks）.Asthma rat model was established by sensitization and stimulation with ovalbumin（OVA） conjuncted with Al（OH）3.The rat airway morphological parameters changes,the gene and protein expression of α-SMA in bron-chopulmonary tissue at different time points were observed by HE staining,RT-PCR and immuno-histochemical method.Result：①The WAt/Pbm,WAi/ Pbm,and WAm/ Pbm were significantly increased in the asthma group on the two weeks,four weeks,and eight weeks compared with that in normal group（all P0.05）,and the WAt/ Pbm,WAi/ Pbm,and WAm/ Pbm were showed the trend of gradually increasing with the days of stimulation,the asthma group on the four weeks was higher than that on the two weeks,the asthma group on the eight weeks was higher than that on the four weeks（all P0.05）.The WAt/Pbm,WAi/ Pbm,and WAm/ Pbm on the two weeks,four weeks,eight weeks in the chaipu decoction-treated group.And that on the two weeks,four weeks in the asthma group were lower than eight weeks in the asthma group（all P0.05）.But that in the all chaipu-treated group were higher than the control group.②The gene and protein expression of α-SMA on the two weeks,four weeks and eight weeks were significantly increased in the asthma group compared with that on the two weeks,four weeks,eight weeks in the chaipu decoction-treated group and control group（all P0.05）,and showed the trend of gradually increasing with the days of stimulation（all P0.05）;there was little expression of the gene and protein expression of α-SMA in the lung tissue of chaipu decoction-treated two weeks group compared with the control group;but all the chaipu decoction-treated groups were increased compared with that in the same week of asthma model group（P0.05）.Conclusion：α-SMA of asthmatic lung tissue is dynamic over expression in the onset of asthmatic.Our study suggest that chaipu decoction involve in the pathogenesis of asthmatic airway remodeling by downregulating the expression of α-SMA inhibiting the airway smooth muscle constriction and hyperplasia.