大蒜3个有效部位配伍对3种人胃癌细胞的杀伤作用

Cytotoxic Effect of Components Formula of Three Garlic Components on Three Human Gastric Carcinoma Cells

目的:观察大蒜3个有效部位组合物对3种人胃癌细胞的杀伤作用,筛选有效部位组合物最佳配伍比例。方法:以大蒜3个有效部位(大蒜油、大蒜总多糖、大蒜总皂苷)为研究对象,选用L16(45)正交表设计分组、给药,以胃癌细胞代谢MTT活力(抑瘤百分率)作为考察指标,采用SPSS软件对实验数据进行处理。结果:大蒜3个有效部位对MKN45细胞杀伤作用的主次顺序为大蒜油(1#)>大蒜总多糖(2#)>大蒜总皂苷(5#),其最佳配比为1#∶2#∶5#=1∶20∶10。大蒜3个有效部位对AGS细胞抑制率影响无明显差别,其最佳配比为1#∶2#∶5#=1∶13.3∶3.3。大蒜总皂苷对人胃癌HGC-27细胞抑制率的影响显著(P<0.01),影响的主次顺序为大蒜总皂苷>大蒜油>大蒜总多糖,其最佳配比为1#∶2#∶5#=1∶200∶50。结论:3种人胃癌细胞对大蒜3个有效部位组合物的杀伤作用的敏感性不同,3个有效部位组合对3种人胃癌细胞杀伤作用的最佳配比浓度亦不相同。

Objective：To investigate the killing effect and determine the optimum proportion of compo-nents formula of three garlic components on three human gastric carcinoma cells.Method： With three garlic effective parts（garlic oil,garlic total polysaccharides,garlic total saponins） as the research object,group and administration in orthogonal test were designed according to L16（45） orthogonal chart.MTT activities of human gastric carcinoma cell metabolism[inhibition rate（%）] were used as indexes and data were analyzed by SPSS software.Result： The killing activity of three garlic effective parts is oil（1#） polysaccharides（2#） saponins（5#）.The optimum proportion of three garlic components on MKN45 cells was 1#∶2#∶5#=1∶20∶10.There was no significant difference of three garlic components in affecting the inhibition rate on AGS cells.The optimum proportion of three garlic components on AGS cells was 1#∶2#∶5#=1∶13.3∶3.3.Garlic total saponins（5#）had significant effect on the inhibition rate of HGC-27 cells（P0.01）.The killing activity of three garlic effective parts was saponinsoilpolysaccharides.The optimum proportion of three garlic components on HGC-27 cells was 1#∶2#∶5#=1∶200∶50.Conclusion： Three human gastric carcinoma cells had different sensitivity to the killing activity of three garlic effective parts.The optimum proportions of three garlic components to three human gastric carcinoma cells were also distinct.