HBx和CEACAM1在乙肝相关性肝癌中的表达及意义

Expression and significance of CEACAM1 and HBx in HBV related hepatocellular carcinoma

目的:探讨HBx和癌胚抗原相关细胞黏附分子1(CEACAM1)在乙肝相关性肝癌中的表达及意义。方法:采用免疫组织化学方法(SP法)检测81例乙肝相关性肝癌组织中HBx及CEACAM1的表达情况,分析HBx和CEACAM1与乙肝相关性肝癌的临床病理特征。Western blot检测人正常肝细胞系QZG、肝癌细胞HepG2及稳定转染HBx的肝癌细胞HepG2(HepG2-X)中CEACAM1的表达。结果:81例乙肝相关性肝癌组织中HBx表达阳性率为74.07%(60/81),CEACAM1表达阳性率为71.60%(58/81),二者与门静脉侵袭、淋巴结转移和TNM分期存在显著相关,HBx与CEACAM1的表达呈负相关(rs=-0.310,P<0.01);在HepG2-X中,CEACAM1蛋白表达水平与肝癌细胞HepG2及人正常肝细胞系QZG相比显著降低。结论:HBx的高表达和CEACAM1的低表达与乙肝相关性肝癌的侵袭、转移密切相关,HBx有可能通过抑制CEACAM1的表达而诱导乙肝相关性肝癌的侵袭与转移。

AIM： To investigate the expression and clinical significance of CEACAMI and HBx in HBV related hepatocellular carcinoma. METHODS： The expression of CEACAM1 and HBx in 81 HBV related hepatocellular carcinoma were detected by immunohistochemistry, and the relationship with clinicopathological features was analyzed. The expression of CEACAMt in the human normal hepatocyte cell line QZG, HepG2 and HepG2-X were detected by Western blot. RESULTS： The positive rate of CEACAM1 and HBx in 81 HBV related hepatocellular carcinoma tissues were 71.60% （ 58/81 ） and 74.07% （ 60/81 ） respectively. The expression of CEACAM1 and HBx were correlated with portal vein invasion, nodal metastasis and TNM staging. The expression of CEACAM1 was negative correlated with HBx（ rs = - 0.310, P〈0. 01 ）. CEACAM1 protein was significantly down regulated in HepG2-X than in HepG2-PC and human normal hepatocyte cell line QZG. CONCLUSION： The higher expression of HBx and lower expression of CEACAM1 are correlated with invasion and metastasis of HBV related hepatocelular carcinoma, CEACAM1 may be a effect molecule in HBV related hepatocarcinogenesis.