摘要
以薏苡(Coix chinensis)种子为实验材料,通过脱脂、水提、Resource S阳离子交换层析和Superdex 75凝胶排阻层析等步骤纯化得到一种新的抗真菌蛋白,并对其抑菌活性进行初步鉴定。SDS-PAGE分析显示该蛋白的分子质量约为28kD。抑菌活性鉴定表明,该蛋白对链格孢霉(Alternaria alternate)、绿色木霉(Trichoderma reesei)和白腐菌(Panus conchatus)3株丝状真菌具有显著的生长抑制活性,并呈现出剂量依赖的特征。本研究建立分离薏苡28kD抗真菌蛋白的方法,制备方法简单易行,所得产品纯度达到95%以上。
A protein from adlay (Coix chinensis) seeds was isolated by degrease with acetone and ether, extraction with 20 mmol/L Tris-HC1 buffer, ion exchange chromatography on Resource S column and size exclusion chromatography on Superdex 75 column. The purity was higher than 95%. Partial characterization and antifungal activity were assayed. The protein exhibited a molecule mass of 28 kD in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This protein could exert potent inhibi- tory activity toward funga/species including Alternaria alternate, Trichoderma reesei and Panus conchatus in a dose-dependent pattern. Antifungal protein has become a popular research topic because of their resistance to pathogenic diseases. In this paper, the studies have demonstrated that adlay seeds have potentially exploitable significance and explored an effective method for the isolation and purification of antifungal proteins from plant seeds.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第5期46-48,共3页
Food Science
基金
国家自然科学基金面上项目(30970611
31171659)
山西省科技攻关计划项目(20100321101)
关键词
薏苡种子
抗真菌蛋白
纯化
adlay seeds
antifungal protein
purification