急性应激对小鼠空间学习记忆功能及脑内GDNF表达的影响

Effects of Acute Stress on Ability of Spatial Learning-memory and Expression of GDNF in Mice Brain

目的:探讨急性应激对小鼠空间学习记忆功能的影响及其机制。方法:采用足底电击的方法建立急性应激模型小鼠,通过Morris水迷宫实验观察小鼠空间学习记忆功能的变化;并且测定小鼠海马和前脑皮层胶质细胞源性神经营养因子的表达。结果:与对照组小鼠相比,在水迷宫实验中,急性应激组小鼠空间学习记忆显著提高(P<0.05);脑内海马CA1区、CA3区、齿状回和前脑皮层GDNF的表达明显增强(P<0.01)。结论:急性应激使小鼠空间学习记忆功能的提高可能与GDNF表达的上调密切相关。

Objective：To study the effects of acute stress on the spatial learning-memory function of mice and its mechanism.Methods：The acute stress model mice with single-factor stressors（foot shock）were applied.The ability of spatial learning-memory of mice were determined by Morris water maze task,and the expression of glial cell line-derived neurotrophic factor（GDNF）in the hippocampus（HP）and prefrontal cortex（PFC）were detected by immunohistochemical method.Results：The ability of spatial learning and memory were significantly enhanced（P0.05）in the stress group mice,compared with the control group mice.The GDNF expression in CA1,CA3 and dentate gyrus of HP and PFC were significantly increased in stress group mice（P0.01）.Conclusion：The acute stress causes the improvement of spatial and memory function in mice which may be nearly related to the up-regulation of GDNF expression.