大鼠感染肝片吸虫后某些免疫介质变化的研究

Changes of Some Immune_mediator in Rats Infected with Fasciola Hepatica

30 只成年大鼠随机分成感染组和对照组,感染组每只大鼠一次口服25 个肝片吸虫囊蚴。通过整体和肝脏离体灌流的方法研究大鼠在感染肝片吸虫后机体免疫功能的变化以及一些免疫介质在抗肝片吸虫和诱导肝损伤中的作用。结果发现,大鼠抗肝片吸虫ES抗原特异抗体(IgG)从感染后第2 周开始显著升高,第8 周回落到与对照组相当水平。感染组大鼠血浆和肝组织匀浆中NO水平虽高于对照组,但差异不显著(p>0 .05),而离体肝脏灌流液中的NO 水平却显著或极显著高于对照组;感染组和对照组的血浆和离体灌流液中的TNF- α水平相当;感染组大鼠血浆cAMP和cGMP水平分别从感染后第8 和6周低于对照组,肝组织中cAMP和cGMP水平也低于对照组。

Abstract Thirty adult rats were randomly divided into infection group and control group. Each rat in infection group was infected orally with 25 F.hepatica metacercariae once. Some immune_mediators were measured after infection with F.hepatica in vivo and vitro. The results showed that serum lgG against ES antigen of Fasciola hepatica started to increase at the first week after infection and returned to the corresponding levels with control group at the 8th week. NO of plasma and liver tissue content was slightly supper to that of control group, but NO levels of infusion isolated liver were apparently higher than that of control group. TNF_α of plasma and infusion fluid had no significant difference between infection group and control group. Plasma cGMP and cAMP in infection rats were less significantly than that of control group at the 8th and 8_10th week of post_infection respectivly.cGMPand cAMP of liver tissue in infection rats were also inferior to control group. Ratio of cAMP to cGMP of rats in infection group also appeared to decreased trend.