鸡传染性法氏囊病三价细胞苗的研究

Research on IBD Tri_Valency Cellular Vaccine

将鸡传染性法氏囊病病毒HBD、HS和HD78 毒株分别适应于鸡胚成纤维细胞,病毒滴度均高达1.0 ×108PFU/ml 以上,以适当比例混合制成鸡传染性法氏囊三价细胞苗。以1.0 ×107PFU/ 只的剂量,经滴鼻、口服途径,分别免疫5 日龄、10 日龄SPF雏鸡,观察一周,无IBD临床症状,迫检可见,法氏囊组织无异常变化。以5.0×105PEU/ 只的剂量,滴鼻、口服免疫10 ～15 日龄雏鸡,7 天后检出琼扩抗体,15 天后血清抗体阳转率达80% 以上,15 天后攻强毒,保护率为100% 。不同疫苗的免疫保护效率比较试验证明,三价细胞苗免疫后的血清抗体阳转率、对强毒的保护率都明显优于双价苗、2512( 国产) 、B2( 美国)、D78(荷兰)、CT(国产)等IBD疫苗,现地应用已逾3000 万羽份。

HBD, HS, HD78 strains of IBDV were adapted to CEF respectively. All the titers of them were high up 1.0×10 8PFU/ml. They were mixed and prepared into tri_valency cellular vaccine. 5_day and 15_day old SPF chicken were vaccinated with this vaccine(1.0×10 7PFU/bird)by intranasal route. These inoculated birds were observed for one week. IBD symptoms have not been seen, and the bursa lesions have not been observed also. 10_day and 15_day old SPF chicken were vaccinated with this vaccine(5.0×10 5PFU/bird) too.The positive antibodies could be checked after 7 day, The antibody positive ratio was high up 80% after 15 days post inoculation. These chickens were challenged with vvIBDV after 15 days post inoculation. The protective ratio was 100%. Immune effection of tri_valency vaccine also was proved better than other IBD vaccines by comparison experiment.