激动素促进受冷害稻苗SOD生物合成机理的探讨

Studies on Mechanism of Kinetin Promoted SOD Biosynthesis of Rice Seedlings after Chilling Injury

实验表明：外施２mg．Ｌ＾－１的激动素（Ｋinetin．ＫＴ）可以提高受冷害（４℃，３６h）稻苗体内ＳＯＤ的活性，而不能提高未受冷害的正常稻苗及无细胞提取液的ＳＯＤ活性 。主了研究ＫＴ促进冷害稻苗ＳＯＤ从头合成机理，本试验采用了转录抑制剂ＡＭＤ（３mg．Ｌ＾－１）自理受冷害稻苗。试验表明，激动素主要是在转录水平上促进ＳＯＤ的从头合成。

Experiments showed that exogenous KT(2 mg. L-1) can increase SOD activity of rice seedlings after chilling injury, but the SOD activity of normal rice seedlings and its cell free extract did not increased with KT treatment. For study on mechanism of de novo SOD biosynthesis of seedling after chilling injury with KT treatement, transcription inhibitor actinomycin D(AMD, 3 mg. L-1 ) was used. The experiments showed that KT promoted SOD de novo biosynthesis mainly on the transciriptional level.