鸡源金黄色葡萄球菌耐药基因定位的研究

LOCATION OF RESISTANT GENE OF STAPHYLOCOCCUS AUREUS FROM CHICKENS

从北京郊区鸡场分离到10株耐药性金黄色葡萄球菌,对这些菌株进行质粒消除,根据质粒消除前后的耐药性检测,发现耐各种药物的抗性基因大多数位于质粒上,少部分抗性基因位于染色体上,由质粒介导的耐药基因占725%,由染色体编码的抗性基因占275%,说明质粒在决定对抗生素的抵抗性中起主要作用。

With high concentration of SDS and elevated temprature (43℃),we succeeded in eliminating plasmids from ten strains of S aureus which were isolated from the farms of Beijing suburb According to the sensitivity test before and after plasmid remoral,we found that 72 5% antibiotic resistance of S aureus was mediated by plasmids,and 27 5% encoded by chromosome The curing test indicated that the plasmids play an important role in coding the resistance to antibiotics