肺炎支原体感染大鼠气道反应性的实验研究

Mycoplasma pneumoniae infection in rat airway reactivity in experimental study

目的:初步探索MP感染与哮喘之间的关系。方法:将40只SD雄性大鼠随机分为四组。采用滴鼻法对实验大鼠进行MP感染,并以1%OVA溶液对其进行雾化吸入,诱导大鼠喘息的发作,观察肺组织形态改变。结果:总阳性率为94.6%,且肺组织可见炎性改变;经1%OVA溶液雾化吸入后,大鼠出现类似支气管哮喘的表现,其BALF中IL-4及IFN-γ含量均升高,IFN-γ/IL-4比值与其他各组比较,差异有统计学意义(P<0.05)。结论:肺炎支原体感染大鼠气道存在慢性炎性反应及高反应状态,经OVA溶液雾化激发后,出现Th1/Th2细胞功能的失衡,加重了气道高反应状态,出现喘息发作的症状。

Objective To research the rat’s reactivity in the airway when Mycoplasma pneumoniae（MP） infections,initially investigated the relationship between MP infection and the bronchial asthma.Methods Forty male SD rats are randomly divided into four groups：MP infection group、MP infection and excitation group、excitation group and normal control group.To infect the rats with MP by the intranasal method,and inhale them with 1% OVA,these methods can deduce the gasp of the rats.After killed,the rats’BALF was leaving for MP-PCR,and detecting the content of IL-4 and IFN-γ;The lung tissues were embedded in paraffin for histological analysis.Results The positive rate by MP-PCR was 94.6% in the rats,which were used intranasal method with MP culture fluid,and the lung tissues were performed the inflammatory changes;after inhaled by 1% OVA,the rats appeared the symptoms like the attacks of Bronchial asthma,and in the model animals’BALF,the content of IL-4 and IFNγis increased,and the ratio of IFN-γ/IL-4 had some significantly difference compare with the other groups（P0.05）.Conclusion The airway of the rats infected by MP,had the performance of chronic inflammatory and hyper responsiveness.After inhaled with 1% OVA,the function of Th1/Th2 cellular immunity are in the state of imbalanced,this change can make more serious of the state of hyperresponsiveness in the airway,and cause the attacks of Bronchial asthma.