微乳液在生物碱薄层色谱分析中的应用

Application of Microemulsions to the Thin Layer Chromatographic Analysis of Alkaloids

以十二烷基硫酸钠-正丁醇-正庚烷-水微乳液作为一种全新的展开剂，进行了41种生物碱的薄层色谱分析。研究结果表明，当微乳液为油包水型和油水双连续型（含水量15％～60％）时，具有与有机溶剂相似的作用，生物碱的Rf值多为0．2～0．8之间，层析结果满意。通过调节徽乳液的含水量，即能获得适宜的展开系统，可用于生物碱的分离鉴定。

The chromatographic behaviour of 41 alkaloids on thin layers using sodium dodecyl sulphate/ n-C4H9OH/n - C7H16/H2O microemlsion as mobile phase has been studied. The Rf values of the alkaloids were determined. The results showed that the W/O microemulsion (containing 15%、 30% water) and the W/O - O/W microemulsion (containing 45 % 、60% water) were realizable mobile phases.