茄子幼苗耐低温性生理机制研究

Physiological Mechanism of Chilling Tolerance in Eggplant Seedling

选择耐低温性不同的6份茄子高代自交系幼苗为材料,进行白天10℃/夜间7℃低温处理10d,调查其耐低温性、生长指标(株高、茎粗、鲜重、干重)、生理指标(POD、SOD、APX活性及脯氨酸含量),以探讨低温对茄子幼苗生长过程中的生长和生理指标的影响。结果显示:(1)各材料的上述性状在常温下均差异不显著,而在低温处理下差异极显著;与对照相比较,幼苗株高、茎粗、鲜重和干重下降,而POD、SOD、APX活性及脯氨酸含量上升。(2)各材料指标的升降幅度与其低温耐受性密切相关,耐低温性强的材料生长指标下降幅度较小,而生理指标提高幅度较大,耐低温性弱的材料则表现相反。(3)各指标间相比较,茄子耐低温性与株高、茎粗、干重、POD活性、SOD活性呈显著正相关,与其余性状的相关未达显著水平。研究表明,低温使茄子幼苗的生长受到一定程度的抑制,但耐低温材料能够通过调节自身的保护酶系统活性来减轻低温的伤害,维持植物体的正常生长及生理代谢功能。

6 materials which were different in tolerance of low temperature were carried on low-temperature treatments,15℃/10℃ in day/night for 2 days,10℃/7℃ in day/night for 10 days. Several characters such as the chilling tolerance, growth indexes （plant height, stalk diameter, fresh weight, dry weight）, physiologi- cal indexes （POD activity, SOD activity, APX activity, proline content） were investigated to study the growth and physiological characteristics under low temperature stress. The results indicated that ： （1） there was no big influence for others characters at normal temperature, the difference was extremely significant for all characters at low temperature. In comparison with CK＇s indexes plant height, stalk diameter, fresh weight, dry weight decreased and POD activity, SOD activity, APX activity, proline content increased after the low temperature treatment. （2）The relationship of chilling tolerance with uplift-descent scale of indexes ranging were osculation. The decrease range of growth indexes of high resisted materials were smaller,the increase range of physiological indexes were larger. While, the medium resisted materials revealed contrary trend. （3）Compared each indexes,chilling tolerance was significant positive correlation with plant height, stalk diameter,dry weight,POD activity,SOD activity,the correlation with others characters did not reach significant level. We can concluded that the growth of eggplant seedlings were negatively influenced by low temperature stress,but resistant materials could adjust themselves to avoid low temperature harms by reg- ulating protective enzyme system,which led to some tolerant ability of eggplant to low temperature condi- tions.