摘要
建立了以滤纸为基质的中草药有效成分芦丁、丹皮酚的固体表面延迟荧光分析法（roomtemperaturedelayedfluorimetry，RTDF）。对影响延迟荧光强度的各种因素进行了详细的研究，实验表明，芦丁的线性范围为5×10^(－6)~1×10^(-4)mol/L，检出限为0．52ng／斑，丹皮酚的线性范围为1×10^(－6)~1×10^(-3)mol/L，检出限为0．08ng/斑。
The solid surface room temperature delayed fluorimetry (RTDF) of rutinum and paeonol, effective compoents in Chinese herbal medicine was established with filter paper as a solid substrate. Meanwile, various factors influencing RTDF intensity were studied in detail. Experiments showed that the linear range of analytical curve was 5 × 10^(-6) ~ 1 × 10^(-4)mol/L for rutinum and 1 × 10^(-6) ~ 1 × 10^(-3)mol/L for paeonol. The detection limit was 0. 52 ng/spot for rutinum and 0. 08 ng/spot for paeonol.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2000年第1期35-37,共3页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金!29875016
山西省青年科学基金!981006
关键词
延迟荧光法
芦丁
丹皮酚
中草药
Room temperature delayed fluorimetry, paper substrate, rutinum, paeonol
