一种新型C_(18)酯型反相高效液相色谱填料的制备

A Novel Method for Preparation of C_(18) Ester-Bond RP-HPLC Packing

发展了一种用于制备反相高效液相色谱填料的实用、经济的方法。以γ环氧丙基氧丙基三甲氧基硅烷为中间偶联试剂,将十八烷基脂肪酸键合至硅胶上。元素分析、色谱测试验证了该工艺的可行性和填料的反相色谱行为。

RP\|HPLC packings have found widespread application in the fields of biology, chemistry and pharmaceutical industry, etc. C 18 bonded phase is one of the most important packing. In order to avoid using toxic and unstable chloro containing silane as derivatization reagent, a new practical and economic procedure had been developed to prepare RP packing. Octadecyl group was bound onto YQG80, 5 μm silica (Tsingdao Oceanic Chemical Factory, particle size d.p.5 μm) with γ glycidoxypropyltrimethoxysilane as coupling agent. Elemental analysis and chromatographic evaluation confirmed the feasibility of the procedure and RP behavior of the packing. After eluting with 500 column volume of mobile phase at different pH (2, 5 and 8), the column still showed good performance (>64 000 theoretical plates/m) and stability.