聚4-甲基-5-乙烯基噻唑键合硅胶柱的选择性研究

Selectivity of Poly (4-Methyl-5-Vinylthiazole) Stationary Phase in Reversed Phase Liquid Chromatography

较为系统地研究了聚4甲基5乙烯基噻唑键合硅胶固定相(PMVCAphase)与C18,C8及苯基柱在反相色谱中甲醇水体系下的选择性差别。结果表明,该固定相与常用反相色谱固定相有相似性,显示了一般反相色谱固定相填料的特性;另一方面,又由于其特殊结构,更多地显示了其在反相色谱上的特殊选择性。

In this paper, the selectivity of poly(4\|methyl\|5\|vinylthiazole) stationary phase (PMV CA phase) is comprehensively studied in reversed\|phase liquid chromatography(RPLC) mode with methanol water system. The selectivity difference between PMV CA phase and three commonly used RPLC stationary phases (C 18 , C 8 and Phenyl phase) is shown. Four typical species of standards (alkylbenzens, chlorobenzenes, nitrobenzenes and PAHs) are selected for the selectivity study. It is shown that the PMV CA phase has some special selectivities: 1) It has different selectivity from C 18 and C 8 phase because the PMV CA phase may form diploe interaction, π electron interaction and hydrogen bonding interaction (with the nitrogen atom and oxygen atom on the phase) with solutes owing to its polar aromatic thiazole ring and polar ester bond etc. on the phase; 2) It has similar property as phenyl phase because both of them can form π electron interaction with solutes. But owing to its polar thiazole ring and other polar group on the phase, which may form dipole interaction and hydrogen bonding interaction with solutes, there are still some differences between these two phases.