反相高效液相色谱法测定实验动物血液和脑组织中川芎嗪

Determination of Tetramethylpyrazine in Animal Serum and Cerebrospinal Fluid by High Performance Liquid Chromatography

用反相高效液相色谱法测定了川芎药材、实验动物血液和脑匀浆中的川芎嗪,方法简便快速。在270nm检测波长下,不经浓缩可检测动物体内1mgL的川芎嗪,川芎嗪在5～500mgL范围内具有良好的线性关系,线性相关系数为0.999。川芎嗪的加标回收率为98%～103%。川芎提取物样品、处理后的动物血清和脑匀浆样品具有较好的稳定性,室温下可放置1星期。

A reversed\|phase high performance liquid chromatographic( RP HPLC ) method for the determination of the tetramethylpyrazine(TMP) in Chuanxiong extract, the animal(mouse) serum and cerebrospinal fluid has been developed. The TMP was separated on an ODS column Zorbax SB C 18 (4.6 mm i.d.×250 mm,5 μm ) at room temperature and detected by using UV detector at 270 nm. The mobile phase was methanol water ( 50∶50, V/V ) containing 0.2 mmol/L of NH 4H 2PO 4 flowing at a rate of 0.8 mL/min and 20 μL samples were injected. The detection limit of TMP was 1 mg/L and the calibration curve is linear between 5 and 500 mg/L with a correlation coefficient (r) of 0.999. The recovery of TMP ranged 98% 103%. The extract of Chuanxiong and pretreated serum and cerebrospinal fluid sample are stable for a week at room temperature.