气相色谱法分离和测定3种植物内源激素

Separation and Determination of Three Plant Internal Hormones by Gas Chromatography

应用大口径毛细管气相色谱法,以正二十二烷为内标物,对植物组织中的吲哚乙酸(IAA)、脱落酸(ABA)和赤霉素(GA)等3种内源激素进行了测定。结果表明:IAA,ABA和GA的最低检测限依次为0.16,0.08和0.48mgL,相对标准偏差均小于3.0%,平均回收率为88.4%～92.2%。方法简便、灵敏、准确、重现性好。

A method for the simultaneous separation and determination of three plant internal hormones indole acetic acid (IAA), abscisic acid(ABA) and gibberellic acid(GA) in plant by gas chromatography with wide\|bore capillary column is described. The gas chromatographic conditions were as follows: FID detector;HP\|1 capillary column,5 m×0.53 mm×2.65 μm film;the column temperature,220 ℃; the injector temperature,250 ℃;the detector temperature,280 ℃,carrier gas,3.5 mL/min N\-2;internal standard n\|docosane. All components and internal standard were separated in 8 min. The detection limit of IAA,ABA and GA were 0.16,0.08 and 0.48 mg/L respectively. The relative standard deviations were 2.2%, 1.7% and 2.8% respectively.The linear range were 0.16\|80 mg/L(r=0.998 6),0.08\|40 mg/L (r=0.999 3) and 0.48 \|240 mg/L (r=0.999 1) respectively. The average recoveries were (88.4±2.4)%,(92.2±1.2)% and ( 91.8 ±1.8)% respectively . The method is simple, rapid, sensitive and reproducible.