贵州省部分少数民族妇女宫颈癌的HPV谱研究

Study on the spectrum of HPV infection in the parts of minority ethnic women with cervical cancer in Guizhou Province

目的探讨人乳头瘤病毒(human papilloma virus,HPV)各亚型在贵州省部分地区少数民族宫颈癌患者中的型别分布情况。方法采用导流杂交基因芯片技术,对145例贵州省少数民族宫颈癌患者石蜡组织标本中的HPV 21种亚型(包括13种高危亚型、5种低危亚型和3种中国人群常见亚型)进行检测,并对HPV感染率及型别分布进行统计学分析。结果145例宫颈石蜡包埋组织HPV总阳性率为35.86%。其中苗族、侗族和土家族患者HPV阳性率分别为27.66%(13/47),38%(19/50),41.67%(20/48)。三个民族HPV感染率差异无统计学意义(χ2=2.177,P=0.337);所有标本HPV 21种亚型中有7种被检测到,均为高危型。HPV感染阳性的亚型从高到低依次为16,58,18,31,52/68,33,感染率分别为65.62%、14.06%、7.81%、4.69%、3.12%、1.56%。三个民族宫颈癌患者石蜡切片HPV亚型分布情况比较,其差异无统计学意义(χ2=0.462,P=0.794)。三个民族中均以HPV 16出现的频率最高,总的百分比为65.6%。HPV单一感染占HPV阳性宫颈癌患者的80.77%。多重感染10例,以HPV 16为主,其中双重感染8例,均为HPV 16合并其它型别的感染;2例三重感染类型均为HPV 16+HPV 18+HPV 31。结论贵州省部分地区少数民族宫颈癌妇女中HPV的感染型别主要为HPV16,58,18,多重感染比例较低,均为HPV 16合并其它型别的感染,体现了本研究少数民族HPV感染的特异性。

Abstract Objective To investigate the general distribution of subtypes of human papilloma virus （HPV） among minority ethnics women with cervical cancer in Guizhou Province. Methods The tech- nology of flow-through hybridization gene chip to detect was adopt on the 21 subtypes HPV of （inclu- ding 13 high-risk subtypes, 5 low-risk subtypes and 3 normal subtypes among Chinese） in 145 paraffin issue sampled from the patients of minority ethnic women with cervical cancer in Guizhou, and was an- alyzed the HPV infectivity and distribution. Results The total HPV positive rate of 145 cervical paraffin embeddings issues was 35.86%, among which the HPV positive rate of patients from Miao nationali- ty, Dong Minority and Tujia ethnic minority were 27.66% （13/47）, 38% （19/50）,41.67% （20/48）, respectively. There were no differences in statistics significance which the HPV infection rate among the three ethnics（X^[2] = 2.177, P = 0. 337）. 7 out of all the HPV21 subtypes samples have been detected as high risk. HPV subtypes with positive infection from high to low were 16,58, 18,31,52/68,33, and infection rate were 65. 62~//0,14.06%,7.81~,4.69~,3.12~, andl. 56~. The were no differ- ences in statistixs significance which the distributions of the paraffin sections among the three ethnic with cervical cancer patients （Xz = 0. 462, P= 0. 794）. The presence rate of HPV16 was the highest a- mong the three ethnics with the total percentage 65.6 %. HPV single infections among the HPV posi- tive patients with cervical cancer were 10, HPV16 as the major part, among which double infections were 8 of HPV16 mixed with other kinds, two kinds of tripling infections were HPV16＋ HPV18A＋HPV31. Conclusion The HPV infection type among the minority ethnic women patients with cervical cancer in Guizhou province are mainly HPV16, 58, 18. The multiple infections were lower proportion and a lot of HPV16 mixed with other types. The results show the specificity of minority ethnic HPV infection in this study.