HBV转基因小鼠血液生理生化指标检测分析

Detection and Analysis of the Blood Biochemical and Hematological Indexes of HBV Transgenic Mice

目的分析乙型肝炎病毒（hepatitis B virus,HBV）基因的整合对转基因鼠代谢和血液指标的影响,为研究乙肝致病机制和新药评价提供可靠的参考依据。方法已传第30代转基因HBV转基因小鼠50只,同窝乙肝表面抗原（HBsAg）阴性小鼠28只,6~8周龄,雌雄各半,眼眶采血,对其血液7项生理和10项生化指标进行品种间和性别统计比较。结果转基因鼠的血糖、尿素氮、肌酐、红细胞、血红蛋白、血小板指标与正常鼠有统计学差异（P〈0.05）,而肝功能、淋巴细胞、脂代谢指标未见统计学差异。结论 HBV转基因鼠有部分生理生化指标改变。

Objective To study the effect of hepatitis B virus（HBV） on the blood biochemical and hematological index of HBV transgenic mice,supply reference for research of pathogenic mechanism and new drug evaluation.Methods Fifty HBV transgenic（at the 30th generation） mice and twenty-eight normal mice in the same family with 6-8 weeks old and females and males half and half were studied.Blood was extracted from the fossa orbitalis.Seven blood biochemical indexes and ten hematological indexes were tested and compared.Results There were significant differences in blood glucose,blood urea nitrogen,creatinine,red blood cell,hemoglobin,platelet between HBV transgenic mice and the normal mice（P〈0.05）.There was no difference between the indexes of liver function,lymphocyte and lipid metabolism.Conclusion Some of the blood biochemical and the blood hematological indexes of HBV transgenic mice are changed.