脂多糖诱发小鼠急性肺损伤microRNA-199a表达及其对基因表达的调控

MicroRNA-199a Expression and Regulation on Gene Expression in Mice with Lipopolysaccharide-induced Acute Lung Injury

目的观察脂多糖(lipopolysaccharide,LPS)诱发的小鼠急性肺损伤中,microRNA-199a(mi R-199a)的表达变化及其对基因的表达调控。方法通过小鼠气道向其肺内注入LPS,构建小鼠急性肺损伤模型,采用Northern blot分析mi R-199a的表达水平;构建mi R-199a的过表达腺病毒,将其转染肺上皮A549细胞,使其过表达;mRNA基因表达芯片分析mi R-199a过表达对基因表达的调控作用。结果 mi R-199a在急性肺损伤病理过程中表达明显降低;mi R-199a主要调控代谢及细胞周期相关基因的表达。结论 mi R-199a在LPS诱发的急性肺损伤病理过程中表达降低,mi R-199a过表达能够促进肺上皮A549细胞中代谢相关基因及细胞周期相关基因的表达。

Objective To observe the expression of microRNA-199a（miR-199a） and its regulation on gene expression of A549 cells in mice with acute lung injury induced by lipopolysaccharide（LPS）.Methods The animal model was established by intratracheal injection of LPS of a dose of 10mg/kg body weight into the lung of the mice.The miR-199a expression was determined by Northern blot.MicroRNA over-expression adenovirus was constructed and used to transfect A549 cells.The gene expression modulation effect of the miR-199a over-expression was tested using mRNA microarray.Results In mice with LPS-induced acute lung injury,the miR-199a expression was significantly down-regulated.MiR-199a could enhance the expression of metabolism and cell cycle related genes.Conclusion MiR-199a is down-expressed in mice with LPS-induced acute lung injury.MiR-199a over-expression can promote the expression of metabolism and cell cycle related genes.